Effect of Intermittent Fe(Ⅱ)-dosed on the Enhancement of Biological Nitrogen and Phosphorus Removal
This study aimed to clarify the effect of intermittent Fe(Ⅱ)addition on nitrogen and phosphorus removal in a ceramic membrane bioreactor(MBR)using synthetic wastewater as the influent.The nutrient removal efficiency,microbial community structure,and the copy number of related functional genes were systematically investigated at Fe/P molar ratios of 1 and 2.The results showed that with Fe(Ⅱ)addition(Fe(Ⅱ)/P=1 and 2),total phosphorus(TP)removal increased by 14.40％ and 18.60％,respectively,while total nitrogen(TN)removal improved by 2.30％ and 6.50％,respectively.The study further examined the effects of Fe(Ⅱ)addition on the dominant bacterial species and the biological nitrogen and phosphorus removal process in activated sludge.The addition of Fe(Ⅱ)enhanced the removal efficiency by increasing the relative abundance of key microbial strains(Dechloromonas,Hyphomicrobium,Nitrospira,Zoogloea,and Acinetobacter)and boosting the copy number of nitrogen removal-related functional genes(AOB-amoA,narG,nirK/nirS,norB,and norZ).Functional predictions using PICRUSt analysis revealed that Fe(Ⅱ)/P=2 treatment promoted the relative abundance of nitrogen removal-related enzymes(NR,NIR,NOR,and N2OR),thereby improving nitrogen removal performance.In conclusion,Fe(Ⅱ)addition at a molar ratio of 2 significantly enhanced biological nitrogen and phosphorus removal by influencing the copy number of key functional genes and altering the microbial community structure.
membrane bioreactorferrousnitrogen and phosphorus removal performancemicrobial community structuredenitrification functional genes
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