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基于主成分和聚类分析的高油花生品种遗传多样性评估及综合评价

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[目的]探究高油花生品种的遗传多样性,并对其进行综合分析评价,以便在生产和花生新品种选育中更好地推广和利用高油花生品种.[方法]以2019-2021年参加河南省花生联合体品种试验的22个高油花生品种为材料,运用SPSS统计软件对其主要农艺、产量和品质等18个性状进行主成分分析和聚类分析.[结果]18个性状遗传基础丰富,变异范围为:0.02~0.33,其中变异系数最大的是油酸含量(0.33),变异系数最小的是脂肪含量(0.02).相关性分析结果表明脂肪含量与蛋白含量呈显著负相关,与荚果产量、籽仁产量呈极显著正相关;蛋白含量与主茎高、侧枝长、百果重、百仁重呈极显著正相关,与生育期呈显著正相关,与每公斤果数、每公斤仁数、出仁率、饱果率、脂肪含量、籽仁产量呈显著负相关;荚果产量、脂肪含量、籽仁产量相互呈极显著正相关.由分析结果可知,高油花生品种脂肪含量与产量有着密不可分的关系,产量性状与农艺性状、品质性状之间均具有一定的影响.运用主成分分析将18个主要性状指标综合为4个主成分影响因子,累计贡献率84.541%,能代表18个性状指标综合评价高油花生品种.聚类分析将22个供试品种分为4大类群,各类群间性状差异较大,第一类:生育期较长,株型较高,分枝、结果较少,籽仁较大,脂肪含量相对较低,蛋白含量相对较高,油酸含量较高,产量较低;第二类:生育期在120 d左右,株型高,分枝、结果较多,籽仁大,脂肪含量较高,油酸含量相对较低,产量最高;第三类:生育期较短,在114 d左右,株型较矮,分枝、结果最多,籽仁较小,出仁率、饱果率高,脂肪含量较高,蛋白含量较低,油酸含量较高,产量较高;第四类:生育期短,在113 d左右,株型矮,分枝、结果少,籽仁较小,出仁率、饱果率高,脂肪含量较高,蛋白含量低,油酸含量较低,产量较高.[结论]果型、产量和脂肪含量、分枝、株高等可作为优质高油花生新品种选择的重要参考指标,22个高油花生品种可聚类为大果早熟高油花生、大果高产高油花生、小果高产高油花生和小果矮杆早熟高产高油花生等4类,为高油花生品种选育和利用提供了参考依据.
Genetic Diversity Assessment and Comprehensive Evaluation of High-Oil Peanut Varieties Based on Principal Component and Cluster Analysis
[Objective]This study aims to investigate the genetic diversity of high-oil peanut varieties and perform a comprehensively analysis and evaluation to facilitate the promotion and utilization of high-oil peanut cultivars in agricultural production and breeding programs.[Method]A total of 22 high-oil peanut varieties from the Henan peanut combination test(2019-2021)were selected for examination.Principal component analysis(PCA)and cluster analysis were applied to 18 key traits including agronomy,yield and quality parameters,utilizing SPSS software for data processing.[Result]A rich ge-netic foundation was observed across the 18 traits with a variation range of 0.02 to 0.33.Oleic acid con-tent exhibited the highest variation coefficient(0.33),while oil content showed the lowest(0.02).Corre-lation analysis indicated that oil content is negatively correlated to protein content,but positively corre-lated with pod yield and kernel yield.The protein content was positively correlated to the main stem height,the lateral branch length,100-pod weight,100-kernel weight,and positively correlated to the growth period,and negatively correlated to the pod number per kilogram,kernel number per kilogram,kernel rate,full pod rate,oil content and kernel yield.Pod yield was positively correlated to oil content and kernel yield.The analysis results showed that the oil content of high-oil peanut was closely related to its yield,and the yield traits,agronomic traits and quality traits had certain effects.Eighteen main traits indexes were synthesized into 4 main component impact factors by using principal component analysis,and the cumulative contribution rate was 84.541%,which could represent 18 traits indexes to evaluate the high-oil peanut varieties comprehensively.The 22 varieties were divided into 4 groups by cluster analysis,and the characters of each group were different.The first category:they had longer growth pe-riod,higher plant type,relatively less branches and pods,big kernel,relatively lower oil content,rela-tively higher protein content,higher oleic acid content and lower yield.The second category:their growth period was about 120 days,and they had high plant type,more branches and pods,bigger kernel,higher oil content,relatively lower oleic acid content,the highest yield.The third category:they had shorter growth period,about 114 days,shorter plant type,the most branches and pods,smaller kernel,high kernel rate and full pod rate,higher oil content,lower protein content,higher oleic acid content and yield.The fourth category:they had the shorest growth period,about 113 days,short plant type,less branches and pods,smaller kernel,high kernel rate and full pod rate,higher oil content,low pro-tein content,lower oleic acid content,higher yield.[Conclusion]Pod type,yield and oil content,branch and plant height can be used as important reference indexes for the selection of high-quality and high-oil peanut varieties,and 22 high-oil peanut varieties can be classified into 4 categories:big-pod,early-maturing and high-oil peanut;big-pod,high-yield and high-oil peanut;small-pod,high-yield and high-oil peanut;small-pod,high-yield and high-oil peanut.This study provides a reference for breeding and utilization of high-oil peanut varieties.

high-oil peanutgenetic diversityprincipal component analysiscluster analysiscomprehensive evaluation

韩艳红、杨海棠、胡延岭、石彦召、朱桢桢、于沐、刘软枝、李盼

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郑州市农业科技研究院,郑州 450005

高油花生 遗传多样性 主成分分析 聚类分析 综合评价

河南优势特色农业产业科技支撑行动计划

20230202002

2024

四川农业大学学报
四川农业大学

四川农业大学学报

CSTPCD北大核心
影响因子:0.657
ISSN:1000-2650
年,卷(期):2024.42(1)
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