Isolation and Identification of The Fungi Parasitizing Coleosporium zanthoxyli in Zanthoxylum armatum and Optimization of Sporulation Conditions
[Objective]To screen the hyperparasite of Coleosporium zanthoxyli and optimize spore produc-tion conditions of the heavy parasitic bacteria,which provided a basis for the biological control of prickly ash rust.[Method]Using morphological characteristics and molecular biology to identify the strain.The single-factor and Box-Behnken response surface methods were used to optimize the spore-producing conditions of strain HC30.[Result]The strain HC30 was screened from the urediospores with rust and found to have hyperparasite effect on C.zanthoxyli,which was identified by morphological and molecular biological methods as Ramularia coleosporii.The optimization results of strain HC30 spore-producing conditions showed that PSA medium was the optimal medium,sucrose was the optimal carbon source,and potassium nitrate was the optimal nitrogen source.The optimal culture conditions were as follows:temperature of 25℃,nitrogen source of 2.5％,carbon source of 2.1％,pH of 7.Under these conditions,the best sporulation of 2.28×108 spores/mL was achieved by the addition of C.zanthoxyli urediospores(0.5 g/L).[Conclusion]The strain HC30 screened from the urediospores with rust has strong hyperpara-site effect on C.zanthoxyli,and the optimal spore production conditions of R.coleosporii are obtained,which provides a theoretical basis for further development of biological agents.
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NSTL
万方数据
维普
