Cloning and Expression Analysis of McXTH Associated with Fruit Softening in Morinda citrifolia
[Objective]This study is to find out the law of noni softening and to clone noni XTH gene and pre-liminarily clarify its expression pattern.[Method]In this study,a fruit firmness tester was used to investi-gate the firmness change of noni fruits in the post-ripening stage under the nature state of room temperature and condition of ethylene treatment.The differentially expressed gene XTH,was screened out from the tran-scriptome data of noni fruits that were picked after 0～48 h.The full length of XTH was isolated after bioinfor-matics analysis.The expression was further analyzed using RT-qPCR.The ethylene release amount was inves-tigated using portable ethylene measuring instrument.[Result]The hardness of the postharvest skin of M.citrifolia in the late ripening stage decreases with increasing storage time.And the softening trend of after ethylene treatment is more significant than when placed naturally at room temperature.The gene XTH(DN16278g1)showing high and largely differential expression was identified from the transcriptome data of M.citrifolia fruits that were picked after 0～48 h.This gene has a complete open reading frame(ORF).It was named as McXTH after clon and sequencing.The sequence of this gene was further submitted to Genebank and the accession number is ON512442.McXTH is 1 062 bp in length encoding 353 amino ac-ids showing 75％～88％of sequence similarity with other plants.RT-qPCR results and endogenous ethyl-ene release amount analysis showed that the endogenous ethylene release trend of M.citrifolia fruits is consistent with the expression of McXTH in the same period.[Conclusion]Ethylene and McXTH take part in regulating fruit softening of M.citrifolia.The cloning of McXTH provides a basis for studying its function in the ripening and softening process of M.citrifolia.
NETL
NSTL
万方数据
维普
