Identification and Functional Prediction of Chicken circ_DNAJC3
[Objective]This study aimed to identify chicken circ_DNAJC3 and predict its potential func-tion in order to lay the foundation for further research on its role in cell biological process.[Method]PCR amplification,agarose gel electrophoresis,Sanger sequencing,subcellular localization,RT-qPCR and bioinformatics software were used to identify the circular structure and stability of circ_DNAJC3,to analyze its location in cells,and its expression level in different tissues,and to predict its potential func-tion and possible modes of action.[Result]Chicken circ_DNAJC3 consists of a stable covalent circular structure from Exons 2 to 4,which is 311 bp.It is conserved in different species,significantly enriched in the cytoplasm(P<0.05),highly expressed in the ileum,thymus,spleen,and cecum(P<0.05)while lower expressed in the hypothalamus and cerebellum(P<0.05).The RNA-binding proteins with which circ_DNAJC3 may interact are AGO2,CELF2,PTBP1 and HNRNPC.Moreover,circ_DNAJC3 can po-tentially interact with gga-miR-132b-5p,and its target genes may participate in cell proliferation,dif-ferentiation,apoptosis,and metabolism through the calcium ion signaling pathway.[Conclusion]These results suggest that chicken circ_DNAJC3 truly exists and may interact with RNA-binding proteins and gga-miR-132b-5p to regulate cellular biological processes.
NETL
NSTL
万方数据
维普
