The Molecular Phylogenetic Relationship of the Ptychobarbus Fish Based on cytb and rag2 Genes
[Objective]The objective of this study is to explore the molecular phylogenetic relationship of the Ptychobarbus and screen molecular markers for species identification.[Method]NJ and Bayesian evolutionary trees of the cytb(5 species)and rag2(P.kaznakovi and P.leptosomus)gene sequences of Ptychobarbus were constructed.Interspecific evolutionary relationships and intraspecific genetic diversity were analyed based on nucleotide polymorphic sites,haplotypes,nucleic acid polymorphisms,genetic distance,genetic differentiation coefficient,and gene flow.[Result]The cytb sequences of each spe-cies were individually clustered into a single branch,indicating that the cytb gene can clearly distinguish the five species of Ptychobarbus.There was a high degree of genetic differentiation(Fst>0.25)among 5 species of Ptychobarbus,and the gene flow between species was weak(Nm<1).The genetic diversity level of P.leptosomus was the highest,while the genetic diversity level of P.conirostris was the lowest.The mo-lecular phylogenetic evolution tree of the rag2 gene showed that P.kaznakovi and P.leptosomus were clus-tered into a single branch.They are independent species.[Conclusion]cytb and rag2 can be used as molecular marker genes for interspecific identification of Ptychobarbus.The above results provide the foundation for the establishment and systematic classification of Ptychobarbus,and the genetic basis for the development of conservation measures for Ptychobarbus.
NETL
NSTL
万方数据
维普
