The Effect of Foliar Spraying of Selenium Fertilizer on the Vitality of Colored Wheat Seeds
[Objective]Colored wheat is a high-quality functional wheat cultivated through distant hybrid-ization and other methods,which has a higher content of nutritional components than ordinary wheat.Im-proving the vitality of colored wheat seeds is of great significance for improving the yield of colored wheat and promoting its industrial development.[Method]This study selected the blue wheat line Shulan Mai 2001 and the purple wheat line Shuzi Mai 1801 as the research objects,with the white wheat line Shumai 691 as the control.Three concentrations of sodium selenite were set for spraying,and the effect of sele-nium on the vitality of colored wheat seeds was determined by measuring the correlation between the vital-ity of colored wheat.[Result]Se2 treatment significantly increased the germination potential,germina-tion rate,and SOD enzyme activity of white wheat and blue wheat by 12.66%,6.90%,14.07%,7.13%,68.70%and 12.82%,9.76%,22.13%,42.00%,37.14%,respectively.The decrease in aging germina-tion rate and conductivity was significantly reduced by 32.43%,12.92%,70.83%,14.00%;Se3 treat-ment significantly increased the germination potential,germination rate,germination index,vitality in-dex,and SOD enzyme activity of purple wheat by 29.17%,14.46%,32.87%,36.97%,46.38%,while the decrease in aging germination rate and conductivity was significantly reduced by 31.67%and 22.70%,enhancing the vitality and storage tolerance of the seeds;Se2 increased the root length and seedling length of white wheat and blue wheat by 8.73%,8.83%,71.20%,14.86%,respectively;Se1 increased the root length of purple wheat seedlings by 2.00%,while Se3 increased the seedling length of purple wheat by 3.72%;Se2 treatment significantly increased the dry weight of wheat seedlings by 9.71%-28.25%;The expression levels of four antioxidant enzyme genes in wheat increased to varying degrees at different stages after flowering.Among them,the relative expression levels of Fe-SOD and Mn-SOD genes were upregulated in the early stage of grain filling,and Cu/Zn-SOD and GR genes were upregu-lated the most in the middle stage of grain filling.The expression levels of SOD related genes and GR genes in colored wheat were higher than those in white wheat throughout the entire grain filling period.[Conclusion]This study indicates that spraying selenium fertilizer can significantly improve the vitality and storage tolerance of colored wheat seeds,cause upregulation of antioxidant enzyme related genes re-lated to seed vitality,and have a greater impact on colored wheat seeds.
NETL
NSTL
万方数据
维普
