摘要
[目的]为发掘更多的抗条纹病青稞种质资源,明确抗条纹病基因在青稞种质资源中分布状况.[方法]选用127份青稞种质资源,利用2个菌株室内人工接种方法进行抗病性鉴定和评价,并利用3个已知抗条纹病基因的SSR分子标记引物进行检测.[结果]127份资源中综合抗病表现免疫、高抗、中抗、高感和中感的分别有13、26、19、52和17份,分别占鉴定材料总数10.24%、20.47%、14.96%、40.94%和13.39%.Rdg基因分子标记检测结果表明,可能携带Rdg1a、Rdg2a和Rdg3的青稞种质资源数分别有3、40和11份,分别占供试材料的2.36%、31.50%和8.66%.[结论]'青0027'可能同时含有Rdg2a和Rdg3这2个抗性基因.
Abstract
[Objective]The aim of this study was to explore a broader range of barley germplasm resources re-sistant to barley leaf strip and to clarify the distribution of barley leaf strip resistance genes within these re-sources.[Method]This study evaluated 127 barley germplasm resources using two strains via indoor artifi-cial inoculation for disease resistance identification and assessment.Three known barley leaf strip resistance genes were detected using SSR molecular marker primers.[Result]The results revealed that among the 127 resources,there were 13,26,19,52,and 17 samples exhibiting immunity,high resistance,medium resis-tance,high sensitivity,and medium sensitivity,respectively.These categories accounted for 10.24%,20.47%,14.96%,40.94%,and 13.39%of the total materials evaluated.Molecular marker detection for the Rdg gene indicated that 3,40,and 11 barley germplasm resources may carry Rdg1a,Rdg2a,and Rdg3 resistance genes,respectively,representing 2.36%,31.50%,and 8.66%of the tested materials.[Conclusion]Among the evaluated germplasm,′Qing 0027′ potentially contains both the Rdg2a and Rdg3 resistance genes.
NETL
NSTL
维普
万方数据