Identification of Leaf Stripe Resistance and Molecular Detection of Resistance Genes of Hulless Barley Germplasm Accessions
[Objective]The aim of this study was to explore a broader range of barley germplasm resources re-sistant to barley leaf strip and to clarify the distribution of barley leaf strip resistance genes within these re-sources.[Method]This study evaluated 127 barley germplasm resources using two strains via indoor artifi-cial inoculation for disease resistance identification and assessment.Three known barley leaf strip resistance genes were detected using SSR molecular marker primers.[Result]The results revealed that among the 127 resources,there were 13,26,19,52,and 17 samples exhibiting immunity,high resistance,medium resis-tance,high sensitivity,and medium sensitivity,respectively.These categories accounted for 10.24%,20.47%,14.96%,40.94%,and 13.39%of the total materials evaluated.Molecular marker detection for the Rdg gene indicated that 3,40,and 11 barley germplasm resources may carry Rdg1a,Rdg2a,and Rdg3 resistance genes,respectively,representing 2.36%,31.50%,and 8.66%of the tested materials.[Conclusion]Among the evaluated germplasm,′Qing 0027′ potentially contains both the Rdg2a and Rdg3 resistance genes.
highland barleybarley leaf stripidentification of disease resistancegene molecular detection
NETL
NSTL
万方数据
维普
