首页|青海省蚕豆赤斑病主要病原菌分离鉴定、生物学特性研究及室内药剂筛选

青海省蚕豆赤斑病主要病原菌分离鉴定、生物学特性研究及室内药剂筛选

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]明确青海省蚕豆赤斑病主要病原菌的种类，寻找适合病原菌生长的环境条件，并筛选出对蚕豆赤斑病病原菌抑制作用较强的药剂品种。[方法]对蚕豆赤斑病病原菌进行形态学和分子生物学鉴定，并进行生物学特性研究，记录其在不同氮源、碳源和温度条件下的生长情况;选用9种化学药剂进行药剂筛选试验，记录在不同浓度、不同药剂下病原菌的生长情况。[结果]前期鉴定出的菌株中，灰葡萄孢出现频率最高，作为试验的代表菌株。生物学特性结果表明葡萄糖和酵母分别作为最适碳氮源、培养温度为20～25℃时，病原菌的生长速度最快。室内药剂筛选试验表明，噁酮·霜脲氰和代森锰锌2种杀菌剂的EC50值较低，EC50值均小于5。00 mg/L，药物毒力较强。[结论]灰葡萄孢是引起青海省蚕豆赤斑病的主要病原菌之一，葡萄糖和酵母对ZS045B生长的促进效果较强，在20～25℃下培养最适合病原菌的生长，噁酮·霜脲氰和代森锰锌对病原菌ZS045B生长的抑制效果较强。
Isolation and Identification of Major Pathogens,Biological Characterization and Indoor Screening of Agents for the Major Pathogenic Fungus of Broad Bean Chocolate Spot in Qinghai Province
[Objective]The objective of this study was to identify the main pathogens causing chocolate spot disease in broad beans in Qinghai Province,determine the environmental conditions conducive to pathogen growth,and screen for chemical agents with potent inhibitory effects against broad bean choco-late spot.[Method]The pathogen responsible for broad bean chocolate spot was identified through mor-phological and molecular biology analyses.The biological characteristics of the pathogen were studied,with particular attention to its growth under varying nitrogen and carbon sources and temperatures.Nine chemical agents were selected for the screening test,and the pathogen′s growth response to different con-centrations and agents was documented.[Result]Among the strains identified in the early stages,Botrytis cinerea was the most prevalent and is considered the representative strain for this study.The pathogen exhibited the fastest growth rate when glucose and yeast extract were used as car-bon and nitrogen sources,respectively,with a culture temperature of 20-25°C.Laboratory screening tests revealed that two fungicides,oxanone-cyanurate and mancozeb,had low EC50 values,both below 5.00 mg/L,indicating strong toxicity.[Conclusion]Botrytis cinerea is confirmed as one of the primary pathogens responsible for broad bean red spot disease in Qinghai Province.Glucose and yeast extract can enhance the growth of the pathogen strain ZS045B,and a culture temperature of 20-25°C is optimal for its growth.Both oxanone-cyanurate and mancozeb demonstrated strong inhibitory effects on the growth of the pathogen ZS045B and are recommended for further field trials.

Qinghai Provincebroad bean chocolate spotbiological characteristicsidentification of pathogenic bacteriascreening of agents

陈华、喻敏博、侯璐、张贵、刘玉皎

展开 >

青海大学农林科学院/青海省农林科学院/青海省农业有害生物综合治理重点实验室/青藏高原生物技术教育部重点实验室,西宁 810016

青海省 蚕豆赤斑病 生物学特性 病原菌鉴定 药剂筛选

"西部之光"人才培养计划现代农业产业技术体系建设项目-国家食用豆产业技术体系建设项目青海省农牧业科技创新平台—蚕豆产业技术转化研发平台项目

CARS-08QNKP-04

2024

10.16036/j.issn.1000-2650.202305248

四川农业大学学报
四川农业大学

四川农业大学学报

CSTPCD北大核心
影响因子：0.657
ISSN：1000-2650
年,卷(期)：2024.42(2)
  • 18