摘要
[目的]探究不同改良方式对豆禾混播草地的丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)群落及区系特征的影响机制.[方法]以云南省种羊场豆禾混播草地为研究对象,调查2019年直接改良(DR)草地与轮作改良(CR)草地,以围封(NG)草地为对照,借助高通量测序、菌丝测定与孢子密度测定技术,测定AMF群落特征.[结果]直接改良草地中孢子密度、球囊霉属(Glomus)显著高于轮作改良方式.孢子密度与土壤硝态氮、土壤微生物氮含量呈显著正相关关系,菌丝长度、菌丝密度与土壤氨态氮含量呈极显著正相关关系,与土壤速效磷含量呈极显著负相关关系.[结论]直接改良方式比轮作改良方式更有利于土壤养分的增加,更有利于AMF与植物共生关系的构建.
Abstract
[Objective]The study aimed to investigate the influence of different grassland improvement practices on plant communities and the distinguishing characteristics of arbuscular mycorrhizal fungi(AMF)in legume-grass mixtures.[Method]High-throughput sequencing and mycelium and spore den-sity measurement techniques were employed to determine the community characteristics of AMF in the di-rectly improved grassland(DR)and the rotationally improved grassland(CR)in 2019.A fenced native grassland(NG)served as the control.[Result]Spore density and the abundance of the genus Glomus were significantly higher in the directly improved grassland(DR)than in the rotationally improved grass-land(CR).There was a significant positive correlation between spore density and both soil nitrate nitro-gen and soil microbial nitrogen.Additionally,a significant positive correlation was observed between soil ammonia nitrogen and both mycelium length and mycelium density.Conversely,there was a significant negative correlation between plant-available phosphorus and both mycelium length and mycelium den-sity.[Conclusion]Direct improvement practices are more conducive to increasing soil nutrients and es-tablishing symbiotic relationships than rotational improvement practices.
基金项目
国家自然科学基金面上项目(32271776)
大学生创新训练计划(2022)(2221998152)
NETL
NSTL
维普
万方数据