The Effect of Different Grassland Improvement Methods on Soil AMF in Grass-Legume Mixtures
[Objective]The study aimed to investigate the influence of different grassland improvement practices on plant communities and the distinguishing characteristics of arbuscular mycorrhizal fungi(AMF)in legume-grass mixtures.[Method]High-throughput sequencing and mycelium and spore den-sity measurement techniques were employed to determine the community characteristics of AMF in the di-rectly improved grassland(DR)and the rotationally improved grassland(CR)in 2019.A fenced native grassland(NG)served as the control.[Result]Spore density and the abundance of the genus Glomus were significantly higher in the directly improved grassland(DR)than in the rotationally improved grass-land(CR).There was a significant positive correlation between spore density and both soil nitrate nitro-gen and soil microbial nitrogen.Additionally,a significant positive correlation was observed between soil ammonia nitrogen and both mycelium length and mycelium density.Conversely,there was a significant negative correlation between plant-available phosphorus and both mycelium length and mycelium den-sity.[Conclusion]Direct improvement practices are more conducive to increasing soil nutrients and es-tablishing symbiotic relationships than rotational improvement practices.
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万方数据
维普
