Response of Fungal Communities in Container Seedling Substrates of Cunninghamia lanceolata to Exponential Fertilization
[Objective]This study was designed to further explore the overall response characteristics of the substrate fungal community of Cunninghamia lanceolata seedlings to exponential fertilization.[Method]A light container nursery substrate and the C.lanceolata clone"Yang 061"was chosen as re-search object.The effects of fertilization treatments with four concentration gradients(EF1,40 mg/plant;EF2,80 mg/plant;EF3,120 mg/plant;EF4,160 mg/plant),as well as a control without any fertiliza-tion,on the physical and chemical properties,fungal community structure,and functional groups of C.lanceolata seedling substrate were studied based on(Illumina MiSeq)high throughput sequencing and FUNGuild fungal function prediction techniques.[Result]Short-term exponential fertilization had no effect on the diversity of substrate fungi,but significantly increased the richness index.Trechisporales and Hypocreales,Sordariales,Chaetosphaeriales and Mortierellales were the dominant orders of the three dominant fungal classes of Agaricta,Coprochia,and Mortierellales,respectively.Exponential fer-tilization significantly increased the relative abundance of Sarcoidales,and significantly decreased the relative abundance of Coproconidiales and Mortierellales.The nutrition types of substrate fungi were pri-marily saprophytic,while woody saprophytic fungi were the dominant functional group.Following expo-nential fertilization,the relative abundance of pathological saprophytic symbiotic transition,saprophytic symbiotic transition and plant pathogenic fungi decreased significantly,while the relative abundance of arbuscular mycorrhizal fungi increased significantly.RDA analysis showed that AP,TK and TN were the main environmental factors affecting the community structure and function of substrate fungi.Exponential fertilization significantly increased the content of AP and TN in the substrate,suggesting that exponential fertilization might promote the reproduction of N-and P-sensitive fungi,which belonged to saprotrophic and symbiotic types.As the amount of fertilization increased,the saprotrophic fungi gradually became dominant,which intern suppressed the growth of symbiotic fungi.[Conclusion]Exponential fertilization effectively improves the availability of substrate nutrients,regulates the dominant community structure of fungi,increases the proportion of symbiotic and saprophytic fungi,reduces the amount of pathogen,which provides good conditions for the growth and development of C.lanceolata seedlings.
NETL
NSTL
万方数据
维普
