首页|日粮中添加益生素对藏羊羔羊生长性能和瘤胃细菌结构与功能的影响

日粮中添加益生素对藏羊羔羊生长性能和瘤胃细菌结构与功能的影响

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[目的]旨在研究日粮中添加0。2%益生素对藏羊羔羊生长发育及其瘤胃细菌多样性的影响。[方法]选择体重相近且健康状况良好的3月龄藏羊60只,随机分为两组,每组30只。对照组饲喂基础日粮(C组),益生素组在基础日粮中添加0。2%产酶益生素(T组)。90 d饲喂试验结束时,每组随机选择6只试验羊屠宰取瘤胃内容物,通过16S rDNA高通量测序技术分析微生物群落的变化。[结果]① T组的终末体重、平均日增重显著高于C组(P<0。05),而C组的料重比显著高于T组(P<0。05);② 两组间Shannon指数、Chao1指数、Simpson指数和Ace(覆盖率)差异均不显著(P>0。05);③ C组的厚壁菌门(Firmicutes)、普雷沃氏菌科(Prevotellaceae)相对丰度显著低于T组(P<0。05),而C组的变形菌门(Proteobacteria)相对丰度显著高于T组(P<0。05)。[结论]日粮中添加0。2%益生素能够明显促进藏羊羔羊的生长性能,并在一定程度上能够增加瘤胃有益菌的丰度。
Effect of Dietary Supplementation of Probiotics on Growth Performance and Rumen Bacterial Structure and Function in Tibetan Lambs
[Objective]The aim of this experiment is to investigate the effects of adding 0.2%probiotics to the diet on the growth and development of Tibetan sheep lambs and their rumen bacterial diversity.[Method]Sixty 3-month-old Tibetan sheep of similar weight and in good health were selected and ran-domly divided into two groups of 30 sheep each.The control group was fed the basal diet(group C),and the probiotic group was supplemented with 0.2%enzyme-producing probiotics to the basal diet(group T).At the end of the 90 day feeding experiment,six test sheep in each group were randomly selected to be slaughtered to collect rumen contents,and analyzed for changes in the microbial communities by 16S rDNA high-throughput sequencing technology.[Result]①The final weight and average daily weight gain of the T group were significantly higher than that of the group C(P<0.05),while the feed-to-weight ratio of the group C was significantly higher than that of the group T(P<0.05);②The differences in Shannon index,Chao1 index,Simpson index and Ace(coverage)between the two groups were not sig-nificant(P>0.05);③The relative abundance of Firmicutes and Prevotellaceae in the group C was signifi-cantly lower than that of the group T(P<0.05),while the relative abundance of Proteobacteria in the group C was significantly higher than that of the group T(P<0.05).[Conclusion]The addition of 0.2%probiotics to the diet can significantly promote the growth performance of Tibetan lambs,and to a certain extent,increase the abundance of beneficial bacteria in rumen.

rumen microorganism16S rDNATibetan sheepproduction performanceprobiotics

周力、侯生珍、吴振岭、闫小兰、桂林生、王志有

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青海大学农牧学院,西宁 810016

瘤胃微生物 16S rDNA 藏羊 生产性能 益生素

青海省科技厅项目

2022NK169

2024

四川农业大学学报
四川农业大学

四川农业大学学报

CSTPCD北大核心
影响因子:0.657
ISSN:1000-2650
年,卷(期):2024.42(2)
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