Effect of Dietary Supplementation of Probiotics on Growth Performance and Rumen Bacterial Structure and Function in Tibetan Lambs
[Objective]The aim of this experiment is to investigate the effects of adding 0.2%probiotics to the diet on the growth and development of Tibetan sheep lambs and their rumen bacterial diversity.[Method]Sixty 3-month-old Tibetan sheep of similar weight and in good health were selected and ran-domly divided into two groups of 30 sheep each.The control group was fed the basal diet(group C),and the probiotic group was supplemented with 0.2%enzyme-producing probiotics to the basal diet(group T).At the end of the 90 day feeding experiment,six test sheep in each group were randomly selected to be slaughtered to collect rumen contents,and analyzed for changes in the microbial communities by 16S rDNA high-throughput sequencing technology.[Result]①The final weight and average daily weight gain of the T group were significantly higher than that of the group C(P<0.05),while the feed-to-weight ratio of the group C was significantly higher than that of the group T(P<0.05);②The differences in Shannon index,Chao1 index,Simpson index and Ace(coverage)between the two groups were not sig-nificant(P>0.05);③The relative abundance of Firmicutes and Prevotellaceae in the group C was signifi-cantly lower than that of the group T(P<0.05),while the relative abundance of Proteobacteria in the group C was significantly higher than that of the group T(P<0.05).[Conclusion]The addition of 0.2%probiotics to the diet can significantly promote the growth performance of Tibetan lambs,and to a certain extent,increase the abundance of beneficial bacteria in rumen.