Resistance Genes Composition in Elite Restorer Taihui 808
[Objective]Taihui 808 is an elite restorer with excellent plant type,quality,and resistance.It is highly marketable and widely used in the breeding of hybrid rice in the Yangtze River Basin.Therefore,clarifying the composition of resistance genes and disease resistance of Taihui 808 can provide timely and effective references for rice breeding and material improvement,promoting and ensuring its safe applica-tion in rice production.[Method]Taihui 808 was studied,using LTH as a negative control and IRBLkm-Ts(a monogenic line containing the resistant gene Pikm)as a positive control.The gene se-quence and expression pattern of the Pikm locus were analyzed by PCR amplification.The disease resis-tance of the tested rice materials was evaluated through inoculation with 38 isolates of Magnaporthe ory-zae.[Result]It identified that Taihui 808 contains the Pikm gene,which is induced by M.oryzae infec-tion and starts upregulating expression after 24 hours post-inoculation.The analysis of rice blast resis-tance discovered that Taihui 808 has 5 pathogenic isolates,which is significantly fewer compared to the monogenic line IRBLkm-Ts.[Conclusion]These results indicate that Taihui 808 may contain new resis-tant genes that confer it a broader spectrum of resistance compared to monogenic line.However,the emer-gence of pathogenic strains suggests a need for timely monitoring and improvement on its disease resis-tance.
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