Optimization of the Genetic Transformation System of Cucumber Mediated by Agrobacterium tumefaciens
[Objective]Screening of Agrobacterium-mediated genetic transformation system for cucumber and laying the foundation for the new variety cultivation through biotechnology.[Method]The cotyledon of the North China type cucumber cultivar'9930'was used as the experimental material.Various factors,including kanamycin concentration(Kan),pre-culture time,addition of acetosyringone(AS)to the pre-medium,pH value of the differentiation medium and addition ofdifferent exogenous substances to the rooting medium,were investigated for their effects on the genetic transformation of cucumber.[Re-sult]The threshold concentration of Kan at the stages of adventitious bud differentiation and rooting was 150 mg/L.The induction rate of cucumber adventitious buds without preculture treatment was the highest and reached its peak at 77.17%.When the pH value of the differentiation medium was 5.6,the highest in-duction rate of adventitious buds was obtained and was 75.26%.The rooting rate of explants on a rooting medium supplemented with 0.5 mg/L of IBA reached 100%,resulting in a significant increase in root length.[Conclusion]In the transgenic transformation system of cucumber cultivar'9930',a concentra-tion of 150 mg/L of Kan can be used as a selection pressure during adventitious bud differentiation and rooting stages.The absence of pre-culture is more favorable for the induction of resistant buds.The suit-able pH value for adventitious bud differentiation medium is 5.6,and 0.5 mg/L IBA is the optimal hor-mone concentration for inducing rooting of explants.
NETL
NSTL
万方数据
