β3-肾上腺素受体在犊牦牛肾周脂肪组织中的表达分布
Expression and Distribution of β3-AR in Perirenal Adipose Tissue of Yak Calves
陈付菊 1全七十六 2范伟峰 1徐伟亮 1李颖帮 3赵宇田 1童依然 1陈杏娣1
作者信息
- 1. 青海大学农牧学院,西宁 810016
- 2. 大通回族土族自治县养殖技术推广中心,西宁 810010
- 3. 青海省农业综合行政执法监督局,西宁 810006
- 折叠
摘要
[目的]探究β3-肾上腺素受体(β3-AR,ADRB3)在犊牦牛寒冷适应中的调控作用.[方法]选取不同日龄(1、7和30日龄)的健康雄性犊牦牛,采用免疫组织化学染色(IHC)、实时荧光定量PCR(qRT-PCR)以及Western blot(WB)对犊牦牛棕色/白色脂肪组织中ADRB3 mRNA及β3-AR蛋白的表达、分布及激素敏感性脂肪酶(HSL)基因mRNA表达进行分析.[结果]IHC结果显示,β3-AR在犊牦牛肾周棕色和白色脂肪细胞中均有分布,且从1～30日龄,β3-AR阳性表达强度显著增加(P<0.05).qRT-PCR结果显示,从1～30日龄,ADRB3和HSL mRNA表达水平显著增加(P<0.05).WB结果显示,从1～30日龄,β3-AR蛋白表达水平显著增加(P<0.05).[结论]ADRB3 mRNA和β3-AR蛋白在犊牦牛肾周棕色和白色脂肪组织中均有表达,且其表达量在白色脂肪组织中高于棕色脂肪组织,说明β3-AR在犊牦牛肾周脂肪组织产热和脂解中均发挥重要的调节作用.
Abstract
[Objective]The aim of this study was to explore the regulatory role of β3-adrenergic receptor(β3-AR,ADRB3)in cold adaptation of calf yaks.[Method]Healthy male calf yaks of different ages(1,7 and 30 days)were selected.The expression and distribution of ADRB3 mRNA,β3-AR protein and the hormone-sensitive triglyceride lipase(HSL)mRNA in the perirenal brown and white adipose tissue of calf yaks were detected by immunohistochemical staining(IHC),Real-time fluorescence quantitative PCR(qRT-PCR),and Western blot(WB).[Result]The IHC results showed that the β3-AR was dis-tributed both in perirenal brown and white adipocytes of yak calves,and the positive expression intensity of β3-AR was significantly increased from day 1 to 30(P<0.05).The results of qRT-PCR showed that the mRNA expression level of ADRB3 and HSL were significantly increased from day 1 to 30(P<0.05).The re-sults of WB showed that the protein expression level of β3-AR were significantly increased from day 1 to 30(P<0.05).[Conclusion]ADRB3 mRNA and β3-AR protein were expressed in both perirenal brown and white adipocytes of yak calves,and its expression level in white adipose tissue were higher than that in brown adipose tissue,indicating that β3-AR plays an important regulatory role in nonshivering thermogen-esis(NST)and lipolysis in perirenal adipose tissue of yak calves.
关键词
犊牦牛/肾周脂肪组织/棕色脂肪组织/白色脂肪组织/β3-肾上腺素受体Key words
yak calf/perirenal adipose tissue/brown adipose tissue/white adipose tissue/β3-adrenergic receptor引用本文复制引用
基金项目
青海省科技厅应用基础研究项目(2024-ZJ-785)
国家自然科学基金(31960686)
出版年
2024
国家科技期刊平台
NSTL
万方数据