Sequence Characterization and Expression Pattern of the AK1 Gene in the Ricefield Eel(Monopterus albus)
[Objective]This study aimed to investigate the sequence characteristics of adenylate kinase 1(AK1)and its function during gonad development in ricefield eel(Monopterus albus).[Method]The AK1 gene sequence of M.albus was cloned and bioinformatic analysis for sequences was carried out.Quantitative Real-Time Polymerase Chain Reaction(qRT-PCR)technology was utilized to detect the ex-pression of AK1 in tissues,gonads at different developmental stages and ovaries after incubation with H2O2.Then,immunohistochemistry was used to determine the location of AK1 in M.albus gonads.[Re-sult]The coding region of AK1 sequence in M.albus was 630 base pairs long,which encodes for 209 amino acid residues.AK1 obtains the conserved NMP and LID structural domains of the AK family,and shows high sequence similarity to homologous genes in other Cyprinid fish species.AK1 mRNA was widely expressed in pituitary gland,eyes,heart,liver,kidneys,intestines,spleen,muscles,blood,ovary,and testis,with the highest expression level in muscle and the lowest expression in blood.Further-more,the expression of AK1 kept the peak at the early vitellogenic stage of gonad in the ovarian develop-mental cycle and in the late intersexual stage gonads of the sexual reversal cycle,while the lowest expres-sion presented in the middle to late vitellogenic stage and in the early intersexual stage of gonads.The AK activity showed a trend of decreasing and then increasing after incubation with H2O2(P<0.05),and the trend of change was more obvious at higher concentrations(P<0.05);whereas the expression of the AK1 was suppressed by H2O2.AK1 was mainly located in the granulosa cells of early vitellogenic stage oo-cytes.[Conclusion]The AK1 gene may involve in the regulation of the gonads development in M.albus,which lays a foundation for revealing biological processes(such as energy metabolism)during gonad devel-opment.
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