Molecular Typing,Antibiotic Resistance Phenotype and Antibiotic Resistance Gene Analysis of Extraintestinal Pathogenic Escherichia coli from Giant Panda Origin
[Objective]This study aimed to clarify the antibiotic resistance and the prevalence of antibi-otic resistance genes of extraintestinal pathogenic Escherichia coli(Ex PEC)of giant panda origin in Sich-uan province,and to provide a reference for the clinical antibiotic use to the giant panda infected by Ex PEC.[Method]The pathogenic bacteria were isolated from the extraintestinal organs of wild dead giant pandas in Mianyang and Yaan,Sichuan Province.16S rRNA gene sequence analysis and Ex PEC multi-plex PCR were used for identification,and then the phylogenetic groups,MLST typing and PFGE typing were performed for the isolated Ex PEC.Kirby-Bauer method and Wafergen Smartchip ultra-high throughput qPCR were used to detect antibiotic resistance phenotypes and analyze antibiotic resistance genes.[Result]9 strains of Ex PEC were isolated,and the phylogenetic groups were respectively B2 and A.MLST and PFGE were divided into 3 molecular types,of which the MLST was of ST555,U10 and ST127 types,and the PFGE was of types Ⅰ,Ⅱ and Ⅲ.9 Ex PEC strains were 100%sensitive to poly-myxin B,imipenem,levofloxacin and nalidixic acid.The detection of 9 types of antibiotic resistance genes(ARGs)such as β-lactam,aminoglycosides and tetracyclines resistance presented that the detec-tion rates of β-lactam and multi-drug resistance ARGs were higher.[Conclusion]The phylogenetic group of the 9 strains isolated and identified was mainly B2 group,and the MLST and PFGE types were mainly ST127(MLST)and I(PFGE).The isolated strains exhibited multidrug resistance,with high de-tection rates of β-lactams and multidrug-resistant ARGs.Antibiotic resistance mechanism is mainly me-diated by antibiotic deactivate and efflux pump.
NETL
NSTL
万方数据
