Identification and Comparative Analysis of Fructokinase Genes in Four Crops
[Objective]Based on the whole genome data,the gene identification and analysis of fructoki-nase(FRK)family in four crops(rice,maize,sorghum and foxtail millet)were conducted to clarify the modes of origin duplication,phylogenetic relationship and the characteristics of sequence and expression divergence.The findings of this study will provide data for the functional research on fructokinase and for enhancing crop yield and quality.[Method]The gene structure,conserved motifs,phylogeny,duplica-tion expansion,and gene expression divergence of fructokinase family members in four crops were ana-lyzed using whole genome search,and intra-and inter-genomic comparisons.[Result]A total of 13,16,14 and 16 FRK genes were identified in rice,maize,sorghum and foxmail millet,respectively.Among these,11 in rice,7 in maize,12 in sorghum,and 13 in foxmail millet were generated by dis-persed duplication,while one pair of genes underwent duplication through ρ whole genome duplication(ρWGD)in rice,sorghum and foxmail millet.Eight WGD genes(one ρWGD and seven mWGD genes)were identified in maize.The gene structures of fructokinase family varied widely between inter-subfamilies,yet remained conserved within intra-subfamilies.The gene expression analysis revealed that orthologs displayed similar expression patterns,while paralogs,especially the gene pairs generated by ρWGD,exhibited divergent expression pattern.Minor differences were observed among paralogs dupli-cated by mWGD.[Conclusion]This study delved into the exploration of genes encoding fructokinase in the genomes of four crops,unveiling insights into duplication modes,gene structures,phylogenetic char-acteristics of sequences,and expression patterns.
gramineous cropsfructokinasegene duplicationsequence and expression divergence
NETL
NSTL
万方数据
