四川山区63份马铃薯亲本材料的SSR指纹图谱构建及遗传多样性分析
Construction of SSR Fingerprints and Genetic Diversity Analysis of 63 Potato Materials in Mountainous Regions of Sichuan Province
李佩华 1张伦 2罗杰 2杜婷婷 2董攀 3彭正松 2刘登才4
作者信息
- 1. 四川农业大学小麦研究所,成都 611130;西昌学院农业科学学院,四川 西昌 615013
- 2. 西昌学院农业科学学院,四川 西昌 615013
- 3. 重庆大学生命科学学院,重庆 400044
- 4. 四川农业大学小麦研究所,成都 611130
- 折叠
摘要
[目的]针对近年筛选出的适宜在四川山区种植的63份马铃薯骨干亲本材料开展遗传多样性研究.[方法]从文献中找到208对多态性较高的引物,提取供试材料的DNA进行PCR扩增.[结果]168对引物出现扩增条带,145对引物具有多态性,7对引物可以完全区分供试材料.[结论]用这7对引物构建SSR指纹图谱,发现63份马铃薯材料间的遗传相似系数为0.48～0.98.遗传相似系数为0.481处将供试材料分为A、B和C 3个类群,0.614处将供试材料分为7个类群,表明供试材料间亲缘关系较远,可为马铃薯的遗传育种杂交亲本组配提供参考.
Abstract
[Objective]This experiment aimed to study the genetic diversity of 63 potato backbone parent materials suitable for cultivation in the mountainous regions of Sichuan Province.[Method]We identified 208 primers with high polymorphism from published literature.DNA was extracted from the 63 potato samples and amplified using PCR.[Result]The analysis revealed that 168 primers produced amplified bands,145 primers exhibited polymorphism,and 7 primers were able to completely distinguish the tested materials.[Conclusion]Utilizing these 7 pairs of primers to construct SSR fingerprints,the genetic similarity coefficients among the 63 potato materials ranged from 0.48 to 0.98.At a genetic similarity coeffi-cient of 0.481,the tested materials were categorized into three groups:A,B and C.At a coefficient of 0.614,the materials were further divided into seven groups.This indicates that the tested materials are genetically dis-tant from one another,providing valuable insights for the genetic breeding of potato hybrid parents.
关键词
马铃薯/SSR标记/遗传多样性分析/DNA指纹图谱Key words
potato/SSR markers/genetic diversity analysis/DNA fingerprinting引用本文复制引用
基金项目
"十四五"四川省薯类育种攻关(2021YFYZ0019)
现代农业产业技术体系四川薯类创新团队(川财教[2019159号])
出版年
2024
NETL
NSTL
万方数据