Genetic Analysis and QTL Mapping of Peanut Root Traits
[Objective]In order to analyze the genetic law of peanut root traits,the related QTL loci af-fecting peanut root traits were explored.[Method]202 recombinant inbred lines(RILs)derived from the parental lines Jihuatian 1 and Jihua 201138,was utilized in conjunction with high-density genetic maps to conduct genetic analysis and QTL mapping of six root traits.[Result]The total root length,root surface area,root volume and root dry weight of Jihuatian 1 were 16.99%～45.10%higher than those of Ji-hua 201138,but the average root diameter and total root tip were 7.14%and 31.27%lower than those of Jihua 201138,indicating that there were significant differences in the root systems of Jihuatian 1 and Ji-hua 201138.Genetic analysis showed that the heritability of the six root traits ranged from 0.734 to 0.826,and the absolute values of kurtosis and skewness ranged from 0.07 to 4.76,indicating that these traits were quantitative traits and controlled by multiple genes.The high-density genetic map constructed using the 48 K chip contains 3 265 SNP markers with a total length of 3 213.26 cM.In addition,QTL analysis detected a total of 11 loci related to peanut root traits,distributed across nine chromosomes in-cluding A06,A07,A08,B01,B02 and B03,with LOD values ranging from 3.53 to 17.48,and a single QTL could explain 2.87%-16.26%of the phenotypic variation of their respective traits.Among them,there were five major QTLs with a phenotypic contribution rate of more than 10%,which were qRA_A07,qRA_A08,qRA_B01,qRA_B02 and qRA_B03_1,among which qRA_B03_1 could be stably detected in three replicates and could explain multiple root traits.[Conclusion]Therefore,qRA_B03_1 has poten-tial application value in the breeding process of improving peanut root traits to improve peanut quality and yield.This study lays a foundation for further fine mapping,molecular-assisted selection breeding,and cloning of key regulatory genes.
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