丝瓜根结线虫抗感品种的根际微生物群落比较分析
Comparative Analysis of Soil Microbial Communities in the Rhizosphere of Resistant and Susceptible Loofah Varieties against Root-Knot Nematodes
李文文 1穆蓉 2王鹤冰 3向华丰 3陆景伟 3陈国康2
作者信息
- 1. 西南大学植物保护学院,重庆 400716;重庆市农业科学院蔬菜花卉研究所,重庆 401329
- 2. 西南大学植物保护学院,重庆 400716
- 3. 重庆市农业科学院蔬菜花卉研究所,重庆 401329
- 折叠
摘要
[目的]探究丝瓜根结线虫与根际土壤微生物群落之间的关系,为探讨根结线虫与土壤微生物的关系提供了重要依据.[方法]首先对2个品种渝瓜砧(YGZ)和感病14(GB14)进行室内外抗性鉴定,然后利用高通量测序技术,分析了2个品种在根结线虫田间侵染发病初期的根际微生物多样性和结构组成.[结果]YGZ为高抗品种、GB14为感病品种,且与感病品种GB14相比,抗病品种YGZ根际细菌的多样性和丰富度较低、而真菌多样性和丰富度更高;在属水平上YGZ与GB14微生物群落的物种丰富度存在显著差异,例如镰刀菌属Fusarium丰富度YGZ较GB14低10.00%,芽孢杆菌属Bacillus YGZ较GB14高4.69%;2个品种的微生物物种组成也存在较大差异,YGZ和GB14独有的细菌物种分别为93个和245个、独有的真菌物种分别为91和70个.[结论]丝瓜根结线虫侵染危害对根际土壤细菌和真菌的种类组成和数量水平均有较大影响.
Abstract
[Objective]This study is aimed to investigate the relationship between root-knot nematodes in loofah Meloidogyne and rhizosphere microbial communities,providing important evidence for exploring the interactions between root-knot nematodes and soil microorganisms.[Method]First,the resistance of two varieties,Yuguazhen(YGZ)and Ganbing 14(GB14),was assessed through indoor and outdoor evalua-tions.Subsequently,high-throughput sequencing technology was employed to analyze the diversity and structural composition of the rhizosphere microbial community at the early stages of root-knot nematode in-fection in both varieties.[Result]YGZ was identified as a highly resistant variety,while GB14 was classi-fied as susceptible.Compared to the susceptible variety GB14,the rhizosphere bacteria in the resistant variety YGZ exhibited lower diversity and richness,whereas the diversity and richness of fungi were higher.At the ge-nus level,significant differences in species richness were observed between the microbial communities of YGZ and GB14;for instance,the richness of the genus Fusarium was 10.00%lower in YGZ than in GB14,while the richness of Bacillus was 4.69%higher in YGZ than in GB14.Additionally,there were marked differ-ences in the microbial species composition between the two varieties,with YGZ having 93 unique bacterial species and 91 unique fungal species,while GB14 had 245 unique bacterial species and 70 unique fungal species.[Conclusion]The infection of root-knot nematodes on loofah significantly influences both the spe-cies composition and abundance of bacteria and fungi in the rhizosphere soil.
关键词
丝瓜/根结线虫/微生物多样性/物种丰度/群落结构Key words
loofah/root-knot nematodes/microbial diversity/species abundance/community structure引用本文复制引用
基金项目
重庆市农业科学院市级财政科研项目(cqaas2023sjczhx010)
重庆市农业科学院市级财政科研项目(cqaas2023sjczqn026)
出版年
2024
NETL
NSTL
万方数据