Comparative Analysis of Soil Microbial Communities in the Rhizosphere of Resistant and Susceptible Loofah Varieties against Root-Knot Nematodes
[Objective]This study is aimed to investigate the relationship between root-knot nematodes in loofah Meloidogyne and rhizosphere microbial communities,providing important evidence for exploring the interactions between root-knot nematodes and soil microorganisms.[Method]First,the resistance of two varieties,Yuguazhen(YGZ)and Ganbing 14(GB14),was assessed through indoor and outdoor evalua-tions.Subsequently,high-throughput sequencing technology was employed to analyze the diversity and structural composition of the rhizosphere microbial community at the early stages of root-knot nematode in-fection in both varieties.[Result]YGZ was identified as a highly resistant variety,while GB14 was classi-fied as susceptible.Compared to the susceptible variety GB14,the rhizosphere bacteria in the resistant variety YGZ exhibited lower diversity and richness,whereas the diversity and richness of fungi were higher.At the ge-nus level,significant differences in species richness were observed between the microbial communities of YGZ and GB14;for instance,the richness of the genus Fusarium was 10.00%lower in YGZ than in GB14,while the richness of Bacillus was 4.69%higher in YGZ than in GB14.Additionally,there were marked differ-ences in the microbial species composition between the two varieties,with YGZ having 93 unique bacterial species and 91 unique fungal species,while GB14 had 245 unique bacterial species and 70 unique fungal species.[Conclusion]The infection of root-knot nematodes on loofah significantly influences both the spe-cies composition and abundance of bacteria and fungi in the rhizosphere soil.
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