Effect of Oxytropis ochrocephala on Endoplasmic Reticulum Stress and Damage in Mouse Liver
[Objective]This study aimed to study the effects of Oxytropis ochrocephala on endoplasmic re-ticulum(ER)stress and damage in mouse liver,thus providing a theoretical basis for the poisoning mechanism of Oxytropis ochrocephala in plateau animals.[Method]In this study,the pathological model of Oxytropis ochrocephala-fed mouse was established.The morphological changes in mouse liver tissue were detected by HE staining.The expressions of ER stress-related genes and proteins(Grp78,Chop,Xbp1,Atf4,Atf6)in mouse liver were detected by RT-qPCR and WB.The experiments were to clarify the effects of Oxytropis ochrocephala on ER stress and damage in mouse liver.[Result]Com-pared to control group,the mouse fed Oxytropis ochrocephala showed pathological symptoms,significant weight loss,decreased liver weight,disordered hepatocyte cord,loose arrangement of hepatic lobules,deformation and necrosis in liver cells,and significant elevation in the expressions of ER stress-related genes and proteins.[Conclusion]In conclusion,Oxytropis ochrocephala can induce ER stress response and damage in mouse liver.
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