Screening and Validation of Reference Genes for Real-Time PCR during'Qiangcuili'Fruit Development
[Objective]To screen suitable internal reference genes for qRT-PCR test in the fruit develop-ment of'Qiangcuili'.[Method]Seven commonly used internal reference genes(18S,Actin,EF1-α,RPL13,RP-Ⅱ,TUB3,EF-2)were selected to analyze the expression stability of pericarp and flesh during the development of'Qiangcuili'.geNorm,NormFinder,BestKeeper and RefFinder were used to assess the most stable internal reference genes.PAL1 and PAL3 gene were further employed to validate the expression stability of reference genes.[Result]The expression of TUB3 was the most stable during the fruit development of'Qiangcuili',which could be used as an internal reference gene for gene expres-sion in the subsequent fruit development period.[Conclusion]Four different internal reference gene sta-bility analysis softwares can be used to screen out the most stable internal reference genes during the de-velopment of plum fruit,and the results can provide reference for the selection of internal reference genes during the development of plum fruit.
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