Molecular Detection of Dwarf Genes in 211 Sichuan Wheat Varieties(Lines)
[Objective]This study investigates the distribution of dwarf genes in wheat varieties(lines)in Sichuan,and assesses the effects of dwarf genes aggregation on plant height,spike length and inter-node length in wheat.[Method]The plant height,spike length and internode length of 211 wheat variet-ies(lines)were measured during the 2022-2023 and 2023-2024 growing seasons.Dwarf genes Rht-B1b,Rht-D1b,Rht5,Rht8,Rht9,Rht10,Rht12,and Rht13 were identified using molecular markers.[Result]The distribution frequencies of Rht-B1b,Rht-D1b,Rht5,Rht8,Rht9,Rht12,and Rht13 among the 211 materials were 81.0％,95.7％,28.4％,51.2％,29.4％,19.9％,and 8.1％,respectively.Rht10 was not detected in any of the materials.A total of 43 different gene combinations were identified.The ranking of culm reduction effects of the dwarf genes was Rht9＞Rht5＞Rht12＞Rht8＞Rht13.The height dwarfization effect of promoted varieties and reserve lines was 9.8％and 9.3％,respectively.The inter-node dwarfization effect value of section 4(base section 2)was 22.27％and 25.91％,respectively.[Con-clusion]The most widely used dwarf genes in wheat in the Sichuan area are Rht-B1b,Rht-D1b and Rht8,and the applications of Rht5,Rht9 and Rht12 are increasing.
万方数据
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