Genetic Analysis and QTL Mapping of Soybean Resistance to Two Types of SMV Virus
[Objective]This study aims to explore the genetic resources conferring resistance to soybean mosaic virus(SMV)and to provide further support for understanding the differences in genetic and mo-lecular mechanisms between resistance to recombinant and non-recombinant SMV.[Method]A recom-binant inbred line(RIL)population,comprising 272 families derived from the cross between Ji Dou 17(resistant)and Sui Nong 14(susceptible),was utilized to determine the genetic basis and molecular mechanisms under two distinct environmental conditions.[Result]Following inoculation of the RIL population with HB-SC3 and HB-RS,the segregation ratio of resistant to susceptible lines was found to fit a 1∶1 ratio according to the Chi-square test(P＞0.05).F1 plants exhibited resis-tance to both HB-SC3 and HB-RS,suggesting that Ji Dou 17 contains a dominant gene confer-ring resistance to both strains.However,the susceptibility grades of families infected with HB-SC3 and HB-RS showed significant shifts,with variations of 4.3％-22.2％and 11.1％-129.63％under different environments,respectively,indicating a substantial environmental impact on the symptoms of plants infected with HB-RS.Additionally,three QTLs were identified through QTL analysis.Notably,qRsmv13 is a stable and dominant QTL that confers resistance to both SMV strains,with LOD values ranging from 23.94 to 40.42 and explaining 35.80％-50.46％of the ge-netic variation.The additive gene effect for resistance to both strains was traced back to Ji Dou 17.Furthermore,the LOD values for the two minor QTLs,qRsmv7 and qRsmv14,were 2.95 and 2.62,respectively,accounting for 2.58％and 2.72％of the phenotypic variation.The addi-tive gene effects for these QTLs also originated from Ji Dou 17.The results further demonstrated that the concentration of HB-SC3 in infected plants was approximately 20 times higher than that of HB-RS,and three PR1 genes associated with necrosis were up-regulated following inocula-tion with HB-SC3.The expression levels of these PR1 genes were significantly higher than those observed with HB-RS,suggesting distinct molecular mechanisms between the two SMV strains.[Conclusion]The primary QTL for soybean resistance to recombinant SMV is qRsmv13,with minor effect loci being qRsmv7 and qRsmv14.There are significant differences in the genetic and molecu-lar mechanisms between resistance to recombinant and non-recombinant SMV strains.
soybeansoybean mosaic virusresistanceQTLnecrosis related gene
万方数据
维普
