GWAS Analysis of Yield and Other Traits in the Self-Fertilization Population of Lentinula edodes"Huxiang F2"
[Objective]The aim of this study is to explore the association of SNPs and candidate genes controlling the agronomic traits of Lentinula edodes.[Method]Whole-genome resequencing was conducted on 100 strains of"Huxiang F2"self-progeny to obtain single nucleotide polymorphism(SNP)data.This data was integrated with phenotypic data of agronomic traits collected during cultivation for a genome wide association study(GWAS).[Result]The primary effect region associated with yield traits was located on chromosome 1 within the interval 2 585 129～4 051 693,containing 1 306 significant SNP loci.Addi-tionally,29 significant SNP loci were identified as associated with the average number of buds per tide,and 199 significant SNP loci were related to the number of days to bud emergence in one tide.For stick quality traits,109 significant SNP loci were linked to the hardness trait,and 29 significant SNP loci were associated with the color conversion trait.Furthermore,12,10,and 2 candidate genes associated with yield traits,mushroom bud traits,and stick quality traits were identified,respectively.[Conclusion]This study marks the first use of GWAS to analyze the single-parent population of L.edodes.It provides effec-tive candidate molecular markers for improving the yield of L.edodes,benefits subsequent molecular-assisted breeding,and lays the foundation for the subsequent fine mapping of functional genes.
fruiting body of Lentinula edodesagronomic traitsgenome wide association study(GWAS)single nucleotide polymorphism(SNP)candidate genes
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