首页|不同改良方式对草地土壤AMF丰度、多样性和群落组成的影响

不同改良方式对草地土壤AMF丰度、多样性和群落组成的影响

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[目的]探究人工混播改良草地不同刈割年限对土壤中丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)与植物共生关系及群落组成的影响。[方法]调查了云南省种羊繁育推广中心刈割1a、3a的草地以及围封草地植物群落、土壤性质、AMF菌丝密度、孢子密度和菌根侵染率,并通过Illumina测序分析了土壤中AMF群落组成。[结果]相比围封草地,土壤中的AMF菌丝密度在刈割1 a处理中显著降低,刈割3a增加了 AMF丰富度。不同刈割草地中AMF群落结构差异显著,Glmous为土壤AMF的优势属,且AMF多样性与植物多样性呈显著正相关。[结论]不同改良方式对草地土壤AMF丰度、多样性和群落组成有显著影响,适当的草地管理利用措施有助于草地生态系统中植被与土壤有益微生物共生关系的维持。
Effects of Different Grassland Management Practices AMF Abundance,Diversity and Community Composition in Grassland Soils
[Objective]This study aims to investigate the effects of different grassland management prac-tices on the symbiotic relationship between plants and arbuscular mycorrhizal fungi(AMF),as well as the composition of the mycorrhizal fungi community in grass-legume mixtures.[Method]The research was conducted at the Yunnan Breeding and Promotion Center of Sheep Breeding in Yunnan Province.The experimental design included three different grassland management practices:a mowed 1-year grass-legume mixture,a mowed 3-year grass-legume mixture,and a fenced grassland.For each management type,assessments were conducted on plant communities,soil properties,AMF mycelium density,spore density,mycorrhizal colonization rate,and AMF community composition.[Result]Compared with the fenced grassland,the mycelial density of AMF in the soil was significantly decreased in the 1-year mow-ing treatment,whereas AMF abundance declined in the 3-year mowing treatment.Mowing treatments did not exert significant effects on the spore density or the mycorrhizal colonization rate of AMF.However,the AMF community structure of AMF exhibited significant variation across different mowing treatments,with Glmous identified as the dominant AM fungal genus in the grassland soil.Furthermore,the diversity of AMF was significantly and positively correlated with plant community diversity.[Conclusion]Differ-ent mowing techniques substantially influence the abundance,diversity,and community composition of AMF within grassland soils.Appropriate grassland management and utilization practices are essential for preserving the advantageous symbiotic relationship between vegetation and beneficial soil microorganisms in grassland ecosystems.

mowingarbuscular mycorrhizal fungisymbiotic relationshiprichnessIllumina sequencing

李旭旭、周冀琼、苏颖颖、弓晋超、王文、李香君、王鹏森、多姝玮、黄婷、马骢毓

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四川农业大学草业科技学院,成都 611130

云南省种羊繁育推广中心,昆明 650000

刈割 丛枝菌根真菌 共生关系 丰富度 Illumina测序

2024

四川农业大学学报
四川农业大学

四川农业大学学报

CSTPCD北大核心
影响因子:0.657
ISSN:1000-2650
年,卷(期):2024.42(6)