首页|察尔汗高盐土层盐分特征及其细菌群落空间分布驱动因子

察尔汗高盐土层盐分特征及其细菌群落空间分布驱动因子

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[目的]研究高盐极端环境土体细菌群落结构及其适应高盐环境的特殊功能,利用这些特殊功能进行高盐生态环境的微生物修复,为探索我国西北寒旱地区盐漠沙化岩土生态环境的微生物修复机理提供新思路。[方法]以察尔汗高盐土体细菌群落结构及其驱动因子为研究目标,采用Omicsmart动态在线分析平台对察尔汗盐湖周围的盐渍土区中的细菌和古菌群落利用16S rRNA基因(V3+V4区),对研究区土体剖面63份样本中的细菌进行高通量测序,确定物种丰度及其门下分布规律,并研究高盐环境因子对细菌的影响。[结果]察尔汗高盐土体细菌群落结构为33门108纲502属,在门层次下的优势物种为Firmicutes(厚壁菌门,62。71%)、Proteobacteria(变形杆菌门,16。83%)和Actinobacteria(放线菌门,5。13%)。[结论]群落结构及其空间分布具有明显的分带性、成层性和圈带性。物种丰富度的主要驱动因子为含盐量、Cl-含量、Na+含量和Mg2+含量,并与含水量、pH都有一定的关系。含盐量及pH的变化与物种丰度的变化呈现负相关。此外,含盐量对土体物种丰度及群落结构会产生重大影响。
Saline Characteristics and Driving Factors of Bacterial Community Spatial Distribution in Qarhan Saline Soil Layer
[Objective]This study investigates the community structure of soil bacteria in high-salinity extreme environment and their special functions that enable adaption to such saline conditions.By utiliz-ing these specialized functions for microbial remediation of high-salinity ecological environments,this re-search aims to provide new insights into the mechanisms of microbial remediation in the salt desert and sandy land ecological environment in the cold and arid regions of Northwest China.[Method]This manuscript examined the bacterial community structure and the factors influencing them in the high-salinity soil of Qarhan.Utilizing the Omicsmart online analysis platform,the study analyzed the bacterial and archaea communities in the saline soil area around Qarhan Saline Lake by using 16S rRNA se-quences(V3+V4 region).A total of 63 soil profile samples underwent high-throughput sequencing to de-termine species abundance and distribution relative to established thresholds.Furthermore,this study in-vestigated the impact of high-salinity environmental factors on bacteria communities.[Result]The bac-terial community structure of the high-salinity soil in the Qarhan area comprised 33 phyla,108 classes,and 502 genera.At the phylum level,the dominant species were Firmicutes(62.71%),Proteobacteria(16.83%),and Actinobacteria(5.13%).[Conclusion]The community structure and its spatial distri-bution exhibited distinct zonation,stratification,and ring patterns.The primary driving factors for spe-cies richness were salinity,Cl-,Na+,Mg2+,which also showed certain relationship with moisture content and pH value.Changes in salinity and pH value were negatively correlated with variations in species abundance.Furthermore,salinity has a significant impact on both species abundance and community structure within the soil.

Qarhan Saline Lakehypersaline soil environmentbacterial community structureabundancedriving factors

白振宇、张文、袁媛、赵媛、王新红、LASHARI Zuhaib ul Hassan

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青海大学土木水利学院,西宁 810016

青海省建筑节能材料与工程安全重点实验室,西宁 810016

青海职业技术大学,西宁 810016

青海大学生态工程学院,西宁 810016

中国电建集团青海省电力设计院,西宁 810008

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察尔汗盐湖 高盐土体环境 细菌群落结构 丰度 驱动因子

2024

四川农业大学学报
四川农业大学

四川农业大学学报

CSTPCD北大核心
影响因子:0.657
ISSN:1000-2650
年,卷(期):2024.42(6)