[目的]探究干热河谷地区生态恢复过程中不同植被类型土壤微生物群落结构的变化特征。[方法]运用高通量测序技术对扭黄茅自然恢复地、车桑子人工灌木林地和银合欢人工乔木林地3种植被类型的土壤微生物群落结构及多样性特征进行研究，并分析环境对其影响。[结果]银合欢、车桑子均能提高放线菌门的相对丰度。银合欢更能提高子囊门菌、毛霉菌门的相对丰度。车桑子有利于提高细菌群落的均匀度和真菌群落的丰富度、多样性以及均匀度，银合欢则会显著降低土壤细菌多样性以及真菌群落的丰富度、多样性和均匀度。同一植被类型下土壤微生物区系呈聚集状态，而不同植被类型下土壤微生物区系则呈分散状态。放线菌门、子囊菌门、担子菌门以及毛霉门下的优势菌属是导致土壤微生物区系差异的主要原因。银合欢的微生物网络结构更为复杂，其群落结构更加稳定。此外，土壤中的pH、水解氮(hydrolyzed nitrogen，AN)是影响车桑子灌丛下细菌群落变化的重要因素，全氮(total nitrogen，TN)是影响银合欢人工林地下细菌群落变化的主要动力。pH、全磷(total phosphorus，TP)是影响车桑子灌丛下真菌群落的驱动因子;全钾(total potassium，TK)对于扭黄茅自然恢复草地的真菌群落生长发育具有促进作用;速效磷(rapidly available potassium，AP)、TK对于银合欢人工林地真菌群落具有积极影响。[结论]不同植被类型在不同程度上改善土壤的微环境，从而影响土壤微生物的群落结构，对于改善金沙江干热河谷中退化生态系统具有重要意义。
Soil Microbial Community Structure of Different Vegetation Types in the Dry-Hot Valley of Jinsha River
[Objective]This study is aimed to explore the variational characteristics of the soil microbial community structure among different vegetation types during the ecological restoration process in Dry-hot Valley regions.[Method]High-throughput sequencing technology was employed to investigate the struc-ture and diversity features of soil microbial community across three vegetation types:the naturally re-stored grassland of Heteropogon contortus,the artificially established shrubland of Dodonaea viscosa,and the artificial forest land of Leucaena leucocephala.Furthermore,the environmental influences on the com-munities were analyzed.[Result]Both L.leucocephala and D.viscosa demonstrated the capacity to in-crease the relative abundance of Actinobacteria.Specifically,L.leucocephala exhibited greater efficacy in increasing the relative abundances of Ascomycota and Mucoromycota.Conversely,D.viscosa contrib-uted positively to the evenness of bacterial communities,as well as the richness,diversity,and evenness of fungal communities.However,L.leucocephala was associated with a significant reduction in the diver-sity of soil bacteria and the richness,diversity,and evenness of fungal communities.Within the same vegetation type,the soil microbial flora exhibited an aggregated state,whereas it displayed a dispersed arrangement across different vegetation types.The dominant genera within the phyla Actinobacteria,As-comycota,Basidiomycota,and Mucoromycota primarily accounted for the observed variations in soil mi-crobial flora.The microbial network with L.leucocephala demonstrated a more intricate structure and a high degree of community stability.Furthermore,soil pH and AN in the soil were significant determinants affecting the changes in bacterial communities under D.viscosa shrubbery,while TN emerged as the primary driving factor influencing the changes in bacterial communities under the artificial forest of L.leuco-cephala.For fungal community,soil pH and TP were identified as the driving factors under D.viscosa shrubbery.Soil TK facilitated the growth and development of fungal communities within the natural resto-ration grassland of H.contortus.Moreover,both AP and TK exerted positive effects on fungal communi-ties in the artificial forest of L.leucocephala.[Conclusion]Various vegetation types differentially enhance the soil microenvironment,thereby influencing the community structure of soil microorganisms.This in-teraction is crucial for the restoration of the degraded ecosystem in the Dry-hot Valley of the Jinsha River.
soil microorganismvegetation restorationhigh-throughput sequencingDry-hot ValleyJinsha River
万方数据
维普
