Differences in Digestive,Antioxidant,Nonspecific Immune and Metabolic Enzyme Activities of Longsnouted longirostris with Different Growth Rates
[Objective]This study aimed to analyze the factors contributing to growth rate discrepancies in Longsnouted longirostris reared in pond culture.[Method]Longsnouted longirostris of identical age and specifications[body length(18.28±1.80)cm,body weight(94.63±27.01)g]sourced from the same progenitors were pond-cultured for 229 days.Subsequently,950 fish were harvested from a single pond.The top 5％largest and smallest individuals based on body weight were selected from this pool as representatives of the fast-growth and slow-growth groups,respectively,each com-prising 50 individuals.From these groups,15 individuals were randomly selected and further subdivided into 3 replicates of 5 individuals each.Measurements included body length,body weight,and the activity levels of digestive enzymes in liver,stomach,midgut,antioxidant enzymes and meta-bolic enzymes in the liver and serum.[Result]The mean body length and weight of the fast-growth group[(26.77±0.45)cm,(277.84±17.80)g]was significantly greater than that of the slow-growth group[(17.73±0.63)cm,(72.73±7.36)g](P＜0.05).In terms of digestive enzymes,the activi-ties of amylase and lipase in the liver were significantly higher in the fast-growth group com-pared to the slow-growth group(P＜0.05).Additionally,activities of amylase,pepsin,lipase and tryp-sin in the stomach were significantly higher in the fast-growth group(P＜0.05).Lipase and tryp-sin activities in the intestine were also significantly higher in the fast-growth group(P＜0.05).Regarding antioxidant enzymes,glutathione peroxidase and catalase activities in the liver were significantly higher in the fast-growth group(P＜0.05),while malondialdehyde content was sig-nificantly lower compared to the slow-growth group.Glutathione peroxidase activity and malondial-dehyde content in the serum were significantly higher in the fast-growth group,whereas catalase activity was significantly lower compared to the slow-growth group(P＜0.05).For nonspecific im-mune and metabolic enzymes,alkaline phosphatase and alanine aminotransferase activities in the liver were significantly higher in the fast-growth group(P＜0.05),and acidic phosphatase and alanine aminotransferase activities in the serum were also significantly higher in the fast-growth group(P＜0.05).[Conclusion]Under the conditions of this experiment,fast-growing Longsnouted longirostris exhibited heightened enzyme activity levels of digestive,antioxidant,nonspecific im-mune and metabolic in specific organs or tissues compared to their slow-growing counterparts.This suggests a superior capacity for digestion,antioxidation,immune and metabolism in fast-growing Longsnouted longirostris and provides valuable insights into the factors contributing to growth disparities.
万方数据
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