The Impact of Anemoside B4 on the Immunization Effect of Diphtheria Vaccine in Young Canines
[Objective]The purpose of this study was to investigate the effects of different levels of Ane-moside B4(AB4)on the immune response of healthy young canines after inoculation with canine distem-per and canine parvovirus,in order to provide a theoretical basis for the clinical application of AB4 in ca-nine vaccines.[Method]In the experiment,thirty clinically healthy puppies aged 6 to 8 weeks were se-lected and randomly divided into a blank group(Group Ⅰ),a vaccine group(Group Ⅱ),and a vaccine+AB4 group(divided into low-,medium-,and high-dose AB4 subgroups,denoted as Group Ⅲ,Group Ⅳ,and Group Ⅴ),with 6 puppies in each group.According to the immunization schedule,the first injection of the combined vaccine for the puppies was recorded as D0,and a booster injection was given 14 days later,for a total of three immunizations.The clinical indicators such as body temperature,heart rate,re-spiratory rate,and body weight of each group of puppies at D0,D7,D14,D21,D28,D35,and D42 were observed and recorded,and the proliferation response of peripheral blood lymphocytes and the titer of antigen-specific antibodies were detected.[Result]①AB4 had no significant effect on body tempera-ture,heart rate,respiratory rate and other clinical indicators of canines(P＞0.05).There was no signifi-cant difference in body weight and weight gain rate between the groups at the same time(P＞0.05).②Dif-ferent doses of AB4 could significantly increase the proliferation activity of peripheral blood lymphocytes in a dose-dependent manner(P＜0.05).③ AB4 could induce a significantly increase the titers of CDV and CPV antigen-specific antibodies(P＜0.05),and the increase was dependent on the dose of AB4.[Conclusion]AB4 has no obvious clinical side effects on young canines'immunity.It can up-regulate the lymphocyte proliferation activity of canines,induce antibody secretion,and enhance the immune response of the bivalent vaccine to canines.It has the potential to be used as a vaccine adjuvant for young canines.
Anemoside B4young caninelymphocyte proliferationvaccine effectantigen specific antibody
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维普
