Value of HPV E6/E7 mRNA and P16/Ki-67 Immunocytochemical Dual Staining in Screening CIN2 Lesions
Objective:To evaluate the value of human papillomavirus infection(HPV)E6/E7 mRNA and P16/Ki-67 immunocytochemical dual staining in the screening cervical intraepithelial neoplasia grade 2(CIN2)lesions.Methods:Ninety-two Patients with cervical inflammation or lesions in our hospital from February 2020 to February 2023 were enrolled,and Patients were classified into groups according to the Pathological findings.Study group(n=45)included CIN2 Patients,while control group(n=47)was consisted of CIN1 and chronic cervicitis.The Positive expression rates of HPV E6/E7 mRNA and P16/Ki-67 alone and in combination were compared between two groups.Logistic regression analysis was utilized to screen the risk factors affecting the diagnosis of CIN2 lesions,and the value of separate and combined test of HPV E6/E7mRNA and P16/Ki-67 in screening CIN2 lesions was evaluated by receiver operating characteristic(ROC)curve.Results:compared with control group,Patients in study group accounted for a larger proportion Positive for HPV E6/E7mRNA(82.22%vs 63.83%),P16/Ki-67(77.78%vs 48.94%)and E6/E7 mRNA and P16/Ki-67 dual staining(84.44%vs 53.19%),with statistical difference(all P<0.05).Logistic regression analysis suggested that HPV E6/E7mRNA and P16/Ki-67 were risk factors for CIN2 lesions(P<0.05).The area under the ROC curve of HPV E6/E7mRNA and P16/Ki-67 dual staining for detecting CIN2 lesions was 0.688(95%CI:0.579～0.798),which was larger than that of separate test of HPV E6/E7mRNA[0.592(95%CI:0.476～0.708)]and P16/Ki-67[0.634(95%CI:0.519～0.748)],with statistical difference(P<0.05).The accuracy,sensitivity and specificity of HPV E6/E7mRNA and P16/Ki-67 dual staining for detecting CIN2 lesions were 68.8%,84.4%and 53.2%,which were higher than 59.2%,82.2%and 36.2%of HPV E6/E7mRNA,and 63.4%,77.8%and 48.9%of P16/Ki-67.Conclusion:HPV E6/E7 mRNA and P16/Ki-67 immunocytochemical staining for detection of CIN2 lesions are of certain value,which can significantly improve the diagnostic efficiency of screening cervical CIN2 lesions.
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