Influence of fluorescence quantification on diagnostic efficiency of Mycoplasma pneumoniae IgM antibody
Objective:To investigate the effect of fluorescence quantification on the diagnostic efficiency of Mycoplasma pneumoniae IgM antibody.Methods:186 children with suspected mycoplasma pneumoniae infection with respiratory tract infection admitted to our hospital from April 2020 to April 2022 were selected as the study objects.After admission,the IgM antibodies of Mycoplasma pneumoniae were detected by fluorescence quantitative Polymerase chain reaction(PCR)and enzyme-linked immunosorbent assay(ELISA).The results of indirect fluorescence immunoassay were used as the"gold standard"to compare the diagnostic results of Mycoplasma pneumoniae IgM antibodies by fluorescence quantitative PCR and ELISA.Results:Among 186 children with respiratory tract infection,102 cases were positive for mycoplasma pneumoniae IgM antibody by indirect fluorescence immunoassay,94 cases were positive for mycoplasma pneumoniae IgM antibody by fluorescence quantitative PCR,and 85 cases were positive for Mycoplasma pneumoniae IgM antibody by ELISA.Compared with ELISA method,the sensitivity and accuracy of PCR were higher,and the missed diagnosis rate of PCR was lower(P<0.05).Conclusion:Fluorescence quantitative PCR can effectively improve the diagnostic efficiency of mycoplasma pneumoniae infection,and provide reference for early clinical diagnosis.
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