Regulation of rhythm and brain iNOS levels in mice by different time-restricted feeding patterns
Objective:To explore the differences in the effects of different time-restricted feeding patterns on mouse rhythms and brain iNOS levels.Methods:Thirty-six SPF male Kunming mice were randomly divided into 3 groups and placed in a rhythm box for 7 days of domestication.The following feeding patterns were performed for 24 days:a.Time-restricted feeding(TRF)in the late stage of rhythm(n=12):only open diet for 9 hours at ZT 15-ZT 0(synchronized with the rhythm);b.TRF in the early stage of rhythm(n=12):only open diet for 9 hours between ZT 1.5-ZT 10.5(not synchronized with the rhythm);c.Free diet(n=12):No dietary restrictions.On the fourth day of domestication,the spontaneous activities of 24 hours were recorded and lasted until the end of TRF.The next day at each of the following time points(ZT 0/6/12/18),three mouse brain tissues were taken from each group,and Western Blot was used to detect the levels of BMAL1 and iNOS proteins.Results:Compared with the free diet group,the rhythmic TRF did not affect the normal activity rhythm of mice but reduced the amplitude of BMAL1 expression and downregulated central iNOS levels(P<0.01).The reverse rhythmic TRF group mice exhibited spontaneous activity rhythm disorder,with higher amplitude of BMAL1 and significantly higher levels of iNOS in the brain compared to the rhythmic TRF group mice(P<0.01).Conclusion:Different time-restricted feeding patterns have different regulatory effects on mice's rhythm and central inflammation.The rhythmic TRF helps maintain a healthy activity rhythm and central iNOS levels,while the reverse rhythmic TRF breaks the normal rhythm.
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