The Inhibitory Effect of Crocin in Combination with Adriamycin on Non-Small Cell Lung Cancer Cells
Objective:To investigate the cytotoxicity of Crocin(CRO)and Adriamycin(ADR),and the effects of different combination regimens on proliferation,migration,and invasion in non-small cell lung cancer cells(A549).Methods:A549 cells were used as the research subject.Cells were treated with different doses of ADR and CRO alone or in combination.Cell proliferation and toxicity were assessed using the CCK-8 assay to determine appropriate drug concentrations for cell treatment,as well as the inhibitory effects on cell proliferation and cytotoxicity at different concentrations.The effects of single and combined treatments on cell migration and wound healing were evaluated using the scratch assay.Cell migration and invasion were examined using the Transwell assay.Results:ADR at a concentration of 1.0 μg·mL-1 significantly reduced cell viability(P<0.001)and inhibited cell invasion and migration in vitro(P<0.05).CRO at a concentration of 2.5 mmol·mL-1 significantly reduced cell viability(P<0.001),with viability decreasing as CRO concentration increased(P<0.001).Low-dose CRO alone resulted in significantly lower cell healing rate at 48 hours compared to the control group(P<0.05),but had no significant effect on cell invasion and migration(P>0.05).Combination treatment with CRO and ADR increased cell death,significantly reduced cell healing rate at 48 hours,and significantly enhanced the inhibitory effect on cell invasion and migration(P<0.001).Conclusion:CRO enhances the inhibitory effects of ADR on proliferation,migration,and invasion of A549 cells,and promotes cell death.The combination of CRO and ADR provides a new approach for the treatment of non-small cell lung cancer.
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