Nucleic acid detection of multiple pathogenic bacteria in community-acquired pneumonia and application value of CRP on prognosis evaluation
Objective:To explore the nucleic acid detection of multiple pathogenic bacteria in community-acquired pneumonia(CAP)and application value of C-reactive protein(CRP)on prognosis evaluation.Methods:The clinical data of 98 CAP patients in the hospital were retrospectively selected from January 2023 to May 2024.The sputum samples were collected for sputum bacteriological examination and nucleic acid detection of multiple pathogenic bacteria.The detection status of pathogenic bacteria by different detection methods and the time required for diagnosis were analyzed.Fasting venous blood was collected on the 1st day,3rd day and 7th day of treatment to determine CRP level.The general data and serum CRP level were compared among patients with different prognosis status,and the application value of CRP on prognosis evaluation was discussed.Results:Among 98 CAP patients,62 cases(63.27%)were positive for pathogenic bacteria detected by sputum bacteriology,and 69 cases(70.41%)were positive by nucleic acid detection of multiple pathogenic bacteria,and there was no statistical significance in the positive detection rate between the two detection methods(χ2=1.128,P=0.288).Taking the sputum bacteriological examination result as the standard,the sensitivity,specificity and accuracy rate of nucleic acid detection of multiple pathogenic bacteria were 91.94%(57/62),66.67%(24/36)and 82.65%(81/98)respectively,and the Kappa value was 0.611,with good consistency.The time required for diagnosis of multiple nucleic acid detection was significantly shorter than that of sputum bacteriological examination(P<0.05).The enrolled patients were divided into survival group(improvement and discharge,n=79)and death group(death,n=19)by means of clinical outcomes.There were no statistical differences in gender,age,course of disease and complications between groups(P>0.05).On the 1st,3rd and 7th days of treatment,serum CRP level in the two groups showed a significant decreasing trend(P<0.05),but the CRP level in survival group was significantly lower than that in death group(P<0.05).ROC curve revealed that the area under the ROC curve,sensitivity,specificity and best cut-off value of CRP in evaluating the prognosis of CAP patients were 0.840(95%CI:0.741-0.939,P<0.05),63.16%,94.94%and 52.99 mg/L respectively.Conclusion:The application of nucleic acid detection of multiple pathogenic bacteria in CAP is highly consistent with the traditional bacterial detection method,and the time required for diagnosis is shorter.Dynamic monitoring of CRP level is of great value in evaluating the prognosis of patients,and it is conducive to ensuring the therapeutic effect.
Community-acquired pneumoniaNucleic acid detection of multiple pathogenic bacteriaC-reactive proteinPrognosisEvaluation
万方数据
维普
