Protective effect of rosmarinic acid on ethanol-induced acute gastric ulcer in mice
Objective:To explore the protective effect of rosmarinic acid on the acute gastric ulcer induced by absolute ethanol in mice and observe gender differences.Methods:A total of 30 Kunming mice were randomly divided into control group(normal saline injection group),gastric ulcer model group,rosmarinic acid low and high dose groups(intraperitoneal injection of rosmarinic acid 4 mg·kg-1,20 mg·kg-1),and esomeprazole treatment positive control group(intraperitoneal injection of 3.03 mg·kg-1),6 in each group,half male and half female.In addition to the model group,the mice in each group were administered prophylactically for 5 days,the mice were gavaged with 0.2 ml of absolute ethanol to establish an acute gastric ulcer model,the behavioral changes of the mice were observed,the mice were anesthetized and sacrificed after 1 hour,the gastric mucosal morphology was observed and the ulcer index was calculated,and the pepsin activity activity was determined by protease activity kit.Results:After modeling,the model group showed behavioral changes such as lethargy and slow movement(up to 90%),while only a small number of low-and high-dose drug treatment groups showed slow behavior(40%),suggesting that rosmarinic acid may have a certain protective effect.Compared with the control group,the gastric ulcer index was significantly increased(P<0.05),the modeling was successful;and the low-dose and high-dose rosmarinic acid groups could significantly reduce the damage of ethanol to the gastric mucosa,reduce the gastric mucosal bleeding and ulcer formation,which was similar to that of the positive control drug,the gastric ulcer index could be significantly reduced compared with the model group(P<0.05),and the effect of the low-dose group was more prominent.Pepsin activity was slightly different but not significantly among the groups(P>0.05).There was no significant difference in the protective effect of rosmarinic acid between female and male mice(P>0.05).Conclusion:Rosmarinic acid has a certain protective effect on ethanol-induced acute gastric ulcer model in mice,and the protective effect is similar to that of esomeprazole,which can reduce the formation of ulcers.
EthanolAcute gastric ulcerRosmarinic acidUlcer index
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