Protective effect of deferiprone on alcoholic-induced myocardial injury in mice
Objective:To investigate the protective effect and mechanism of deferiprone on alcohol-induced myocardial injury.Methods:Twenty-four male C57/BL6J mice were adaptively fed for 1 week and randomly divided into a control group,a model group,and a deferiprone intervention group using a random number table method,with 8 mice in each group.An alcoholic myocardial injury model was established by orally administering 10 mL·kg-1·d-1 of 50% alcohol daily.After 1 hour,the intervention group was given deferiprone solution(100 mg·kg-1·d-1)by gavage,while the control group and model group were given equal doses of physiological saline.After 12 weeks,the pathological changes in heart tissue were observed by H&E staining.The levels of lactate dehydrogenase(LDH)and Fe2+in mouse serum and myocardial tissue were detected by microplate assay,and the content of malondialdehyde(MDA)in myocardial tissue was detected by thiobarbituric acid assay.Results:Compared with the control group,the model group mice showed myocardial fiber rupture and dissolution,significant widening of extracellular spaces,infiltration of inflammatory cells,and elevated levels of LDH and Fe2+in serum and myocardial tissue.The content of myocardial MDA significantly increased compared to the control group(P<0.05).After intervention with deferiprone,myofibril fiber rupture,dissolution,extracellular space,and a small amount of inflammatory cell infiltration were significantly improved.The levels of serum and myocardial tissue LDH,Fe2+content,and myocardial MDA content were significantly reduced compared to the model group(P<0.05).Conclusion:Deferiprone has a protective effect on alcoholic myocardial injury in mice,and its mechanism may be related to inhibiting myocardial cell iron overload and reducing alcohol induced oxidative stress damage.