摘要
为了解甲基托布津(Methyltobuzine,TM)和多菌灵(Carbendazim,BCM)对水生动物的毒性效应,在水温(25±2)℃下,将平均体长(6.3±0.7)cm和平均质量(2.4±0.5)g的 6 月龄泥鳅(Misgurnus anguillicaudatus)置于 65 mg·L-1、85 mg·L-1、112 mg·L-1、146 mg·L-1、191 mg·L-1 TM和 26 mg·L-1、34 mg·L-1、45 mg·L-1、58 mg·L-1、76 mg·L-1 BCM下,采用半静水实验法,研究TM和BCM的 24 h、48 h、72 h、96 h的半致死浓度(LC50)和 24 h安全浓度(SC);并在 0.05 mg·L-1、0.50 mg·L-1、5.00 mg·L-1 TM和 0.02 mg·L-1、0.10 mg·L-1、0.50 mg·L-1、2.50 mg·L-1 BCM中胁迫 7 d、14 d、21 d、28 d,检测泥鳅肝脏过氧化物酶(POD)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)活性变化.结果表明,TM对泥鳅的 24 h、48 h、72 h、96 h LC50 分别为 35.48 mg·L-1、26.98 mg·L-1、17.25 mg·L-1 和10.03 mg·L-1,24 h SC为 6.16 mg·L-1;BCM对泥鳅的 24 h、48 h、72 h、96 h LC50 分别为 23.53 mg·L-1、17.66 mg·L-1、6.54 mg·L-1 和 3.58 mg·L-1,24 h SC为 3.98 mg·L-1;TM和BCM胁迫下POD、GOT、GPT活性随浓度升高呈现先上升后下降的趋势,随着胁迫时间延长各参数的活性呈下调趋势.BCM处理组较TM处理组变化趋势更显著.综上所述,TM对 6 月龄泥鳅具有低毒性作用,BCM对 6 月龄泥鳅则具有中等毒性作用;一定剂量TM和BCM长期胁迫对泥鳅造成严重影响,对POD、GPT、GOT活性具有明显抑制作用,随胁迫时间延长抑制加剧,其中BCM较TM抑制作用更强.
Abstract
In order to understand the toxic effects of Methyltobuzine(TM)and Carbendazim(BCM)on aquatic animals,6-month-old loach(Misgurnus anguillicaudatus)with average body length of(6.3±0.7)cm and body weight of(2.4±0.5)g were exposed to TM concentrations of 65 mg·L-1,85 mg·L-1,112 mg·L-1,146 mg·L-1 and 191 mg·L-1 and BCM concentrations of 26 mg·L-1,34 mg·L-1,45 mg·L-1,58 mg·L-1,and 76 mg·L-1 at water temperature of(25±2)℃,using semi-static water test method to probe into the median-lethal concentration(LC50)and 24-h safe concentration(SC)of TM and BCM at 24 h,48 h,72 h,and 96 h.Then,changes in the activities of liver peroxidase(POD),alanine aminotransferase(GPT),and aspartate aminotransferase(GOT)were detected in the 6-month-old loach exposed to 0.05 mg·L-1,0.50 mg·L-1,and 5.00 mg·L-1 TM and 0.02 mg·L-1,0.10 mg·L-1,0.50 mg·L-1 and 2.50 mg·L-1 BCM stress for 7 d,14 d,21 d and 28 d.The results showed that the LC50 of TM for loach was 35.48 mg·L-1 in 24 h,26.98 mg·L-1 in 48 h,17.25 mg·L-1 in 72 h and 10.03 mg·L-1 in 96 h,with the 24 h SC of 6.16 mg·L-1.The LC50 of BCM to loach was 23.53 mg·L-1 in 24 h,17.66 mg·L-1 in 48 h,6.54 mg·L-1 in 72 h,and 3.58 mg·L-1 in 96 h,with the 24 h SC of 3.98 mg·L-1.The activities of POD,GOT and GPT were increased first and then decreased with the increase in concentrations of TM and BCM,showing a downward trend with the extension of stress time,and the more significant change trend of BCM in treatment groups than that in TM treatment groups.In summary,TM had low toxic effect on 6-month-old loaches,while BCM had moderate toxic effect on 6-month-old loaches.Long-term stress with a certain dose of TM and BCM led to serious effect on loach,and the significant inhibition of the activities of POD,GPT and GOT,BCM having stronger inhibitory effect than TM.
基金项目
陕西省自然科学基础研究计划面上项目(2021JM-417)
延安市科技惠民计划(2017HM-05)
NETL
NSTL
万方数据