Effects of Fungicides Methyltobuzine and Carbendazim on Acute Toxicity and Liver Enzyme Activities of Loach
In order to understand the toxic effects of Methyltobuzine(TM)and Carbendazim(BCM)on aquatic animals,6-month-old loach(Misgurnus anguillicaudatus)with average body length of(6.3±0.7)cm and body weight of(2.4±0.5)g were exposed to TM concentrations of 65 mg·L-1,85 mg·L-1,112 mg·L-1,146 mg·L-1 and 191 mg·L-1 and BCM concentrations of 26 mg·L-1,34 mg·L-1,45 mg·L-1,58 mg·L-1,and 76 mg·L-1 at water temperature of(25±2)℃,using semi-static water test method to probe into the median-lethal concentration(LC50)and 24-h safe concentration(SC)of TM and BCM at 24 h,48 h,72 h,and 96 h.Then,changes in the activities of liver peroxidase(POD),alanine aminotransferase(GPT),and aspartate aminotransferase(GOT)were detected in the 6-month-old loach exposed to 0.05 mg·L-1,0.50 mg·L-1,and 5.00 mg·L-1 TM and 0.02 mg·L-1,0.10 mg·L-1,0.50 mg·L-1 and 2.50 mg·L-1 BCM stress for 7 d,14 d,21 d and 28 d.The results showed that the LC50 of TM for loach was 35.48 mg·L-1 in 24 h,26.98 mg·L-1 in 48 h,17.25 mg·L-1 in 72 h and 10.03 mg·L-1 in 96 h,with the 24 h SC of 6.16 mg·L-1.The LC50 of BCM to loach was 23.53 mg·L-1 in 24 h,17.66 mg·L-1 in 48 h,6.54 mg·L-1 in 72 h,and 3.58 mg·L-1 in 96 h,with the 24 h SC of 3.98 mg·L-1.The activities of POD,GOT and GPT were increased first and then decreased with the increase in concentrations of TM and BCM,showing a downward trend with the extension of stress time,and the more significant change trend of BCM in treatment groups than that in TM treatment groups.In summary,TM had low toxic effect on 6-month-old loaches,while BCM had moderate toxic effect on 6-month-old loaches.Long-term stress with a certain dose of TM and BCM led to serious effect on loach,and the significant inhibition of the activities of POD,GPT and GOT,BCM having stronger inhibitory effect than TM.
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