Immunotoxicity of Sodium Dodecyl Benzene Sulfonate Exposure in Water to Yellow Catfish Pelteobagrus fulvidraco
To assess the toxic effect of sodium dodecyl benzene sulfonate(SDBS)on yellow catfish Pelteobagrus fulvidraco,yellow catfish with body weight of(101.4±16.5)g was raised in a flowing water aerated tank and exposed to SDBS at concentrations of 0.0 mg/L,0.2 mg/L,0.4 mg/L,0.6 mg/L,and 0.8 mg/L at a water temperature of(24.5±0.3)℃ for 10 d.Then the activities of serum lysozyme(LZM)and alkaline phosphatase(AKP)were measured,the activities of superoxide dismutase(SOD)and catalase(CAT),the contents of malondialdehyde(MDA),immunoglobulin M(IgM)and complement C3(C3),as well as the total antioxidant capacity(T-AOC)were determined in hepatopancreas.The relative expression levels of complement C3 and CA T genes in the spleen were measured by fluorescence quantitative PCR.The results showed that the levels of complement C3 and immunoglobulin and activities of SOD and LZM and T-AOC were found to be decreased in the hepatopancreas of yellow catfish exposed to SDBS,while the levels of malondialdehyde to be increased.mRNA transcript levels of complement C3 and CA T genes in spleen of yellow catfish showed down-regulation after SDBS exposure,and the mRNA transcript levels of complement C3 gene were significantly down-regulated in the high concentration exposure group(0.8 mg/L)(P＜0.05)and CA T gene mRNA transcript levels were significantly down-regulated in the 0.4-0.8 mg/L exposure(P＜0.05).The findings indicated that the exposure to 0.4-0.8 mg/L concentration of SDBS led to reduce the antioxidant capacity and immune level of yellow catfish.
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