Effects of Air Exposure on Respiratory Metabolism,Energy Metabolism and Antioxidant Capacity of Adult Chinese Mitten Handed Crab Eriocheir sinensis
To investigate the effects of different air exposure stress periods on respiratory metabolism,energy metabolism and antioxidant capacity of adult Chinese mitten handed crab(Eriocheir sinensis),The male crab with body weight of(127.07±10.92)g and female crab with body weight of(86.16±6.80)g were exposed to air for 0 h(control group),8 h,16 h or 24 h,and 12 crabs at room temperature(20-22 ℃),and then the hepatopancreas and muscle were sampled in 6 males and 6 females for determination of the indices of respiratory metabolism,energy metabolism and antioxidant capacity,including activities of lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),malate dehydrogenase(MDH)and catalase(CAT),contents of pyruvate,and malondialdehyde(MDA),total antioxidant capacity(T-AOC)and liver/muscle glycogen ratio,The results showed that(1)the activity of hepatopancreas lactate dehydrogenase(LDH)in male crabs was decreased first and then increased with increase in air exposure period,with the maximal value at 24 h.There was significant decrease in activity of LDH in muscle of both male and female crab,while the activity of succinate dehydrogenase(SDH)was found to be first increased and then decreased,with the maximal level in 8 h.(2)The glycogen content in the hepatopancreas of male crab was shown to be significantly increased with increase in air exposure period.The pyruvate content in hepatopancreas of both male and female crabs was decreased first and then increased,with the maximal level at 24 h,and the maximal glycogen content in the muscle of male crab in 24 h,while the activity of muscle malate dehydrogenase showed a significantly decreasing trend.(3)The maximal T-AOC and CAT activities were observed in the hepatopancreas of female and male crabs at 24 h,significantly higher than those at other time,the maximal malondialdehyde(MDA)content in the hepatopancreas of female crabs in 8 h,and the maximal hepatopancreas MDA content of male crabs in 24 h.The muscle MDA content,T-AOC and CAT activities were decreased with increase in air exposure period.In conclusion,female and male crab had differences in air exposure stress resistance,and the air exposure stress within 24 h has a significant impact on the respiratory metabolism and antioxidant capacity of adult Chinese mitten handed crab,but the energy metabolism was basically unchanged,so the crabs could adapt to the waterless transportation for 24 h by regulating their own physiological metabolism.
万方数据
维普
