Genetic Structure Analysis of Xenocypris davidi Cultured Population Based on Mitochondrial COⅠ Gene Sequence
In order to understand the effects of artificial breeding and selective breeding on genetic diversity of Xenocypris davidi,the genetic structure was investigated in 128 samples of four cultured 1-2 years old populations of X.davidi including Liling(LL)from Hunan,Changxing(CX),Shuangpu(SP)and Wuxing(WX)from Zhejiang in China by using mitochondrial COⅠ gene.The results showed that 75 variable sites were detected in the 539 bp partial sequence of COⅠ,including 74 parsimony informative sites.There was significantly higher content of A+T(56.1％)than that of G+C(43.8％),with obvious(A+T)preference.Eleven haplotypes were defined in 128 individuals,with the dominant Haplotype Hap-01(43 individuals).The results of genetic diversity test showed that the haplotype diversity(Hd)was found to be ranged from 0.226 to 0.605,and the nucleotide diversity(π)from 0.001 0 to 0.022 2,showing different levels of genetic diversity in the four populations.The genetic distance was ranged from 0.014 5 to 0.098 6 among different populations,with the closest genetic distance between CX and WX populations and the longest genetic distance between LL and SP populations.AMOVA analysis revealed that the genetic differentiation coefficient Fst between populations was 0.838 5(P＜0.01),the genetic variation primarily found between populations.NJ phylogenetic tree and genetic distance analysis showed that there was a high genetic differentiation among the four populations.The finding provides basic data for the comparative investigation of genetic diversity changes of X.davidi,and for the proliferation and release of X.davidi and resource protection.
Xenocypris davidigenetic diversitygenetic structurecultured population
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