Analysis of Intestinal Flora and Resistance Gene Composition in Southern Catfish(Silurus meridionalis)Based on Metagenomic Sequencing
To understand intestinal microflora structure and composition of resistance genes of cultured southern catfish Silurus meridionalis,six different species of bacteria were selected from metagenomic sequencing technology and bioinformatics methods for isolation.The results showed that the intestinal flora was annotated to 47 phyla,1 425 genera,and 4 171 species,with dominant species Plesimonas shigelloides,and the relative abundances in 3 samples were 23.3％,23.4％,and 26.4％,respectively.KEGG analysis showed that the genes of southern catfish intestinal flora were primarily involved in carbohydrate metabolism and amino acid metabolism.GO analysis showed that the genes in gut flora of southern catfish were mainly involved in metabolic processes,cellular component and catalytic reactions.Moreover,resistance gene annotation results showed 5 151 resistance genes were identified,and annotated to 31 antibiotic types,without annotation to specific antibiotics in 7 resistance genes.Six species of bacteria were successfully isolated,cultured and purified:Plesiomonas shigelloides,Aeromonas hydrophila,Aeromonas veroni,Aeromonas sobria,Bacillus licheniformis,Lactococcus lactis.The findings provided preliminary analysis of intestinal microflora structure and preliminary statistics of resistance gene carrying in southern catfish,aiming to provide reference data for the prevention and control of diseases in southern catfish culture.
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