Effects of Astaxanthin on Antioxidant Parameters of Zebrafish Embryos Induced by Cd2+
A total of 100 1-hour-old embryos of zebrafish(Danio rerio)were placed in a 120 mm Petri dish,exposed to dimethyl sulfoxide(DMSO,at concentration of＜0.02％,control group),50 μg/L Cd2+(low exposure group),500 μg/L Cd2+(high concentration exposure group),and astaxanthin(AST)combined group(50 μg/L Cd2++100 μg/L AST and 500 μg/L Cd2++100 μg/L AST)at(28.0±0.5)℃.Then the contents of reactive oxygen species(ROS),malondialdehyde(MDA)and activities of glutathione(GSH),superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)were determined in zebrafish juveniles 120 h after administration to evaluate the antioxidant response of zebrafish embryos exposed to heavy metal cadmium ions(Cd2+)and the alleviation effect of AST on oxidative damage in zebrafish.The results showed that Cd2+induced oxidative stress and cause oxidative damage in zebrafish bodies.the ROS and MDA contents were found to be increased with the increase in concentration of Cd2+;and the activities of GSH,SOD and T-AOC to be decreased,with the increasing concentration of Cd2+in zebrafish juveniles exposed Cd2+during the embryonic period for 120 h.After AST was added,the ROS and MDA contents of zebrafish bodies were shown to be decreased compared to those in the Cd2+treatment group,and activities of the GSH,SOD and T-AOC to be increased,indicating that the effects of the Cd2+exposure on antioxidant parameters in zebrafish body was mitigated by the addition of AST.The findings provide more theoretical basis for astaxanthin as a new fish feed additive in ecological aquaculture.
heavy metal Cd2+astaxanthinzebrafish embryooxidative stress
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