Effects of Different Salinity and Light Intensity on Growth of Free-living conchocelis of Pyropia acanthophora
In order to explore the optimal salinity and light intensity conditions for the culture of Py.acanthophora free filaments,we induced free filaments from thallus collected from Tongguling,Wenchang,Hainan Province,and crushed them into 150-200 μm segments.They were then placed in conical flasks and cultured for 29 d under the condition of 12L∶12D at 20 ℃.The growth of free filaments were observed under five salinity gradient groups(15,20,25,30,35)at the illumination intensity of 30 μmol·m-2·s-1,and six illumination intensity gradient groups(20 μmol·m-2·s-1,30 μmol·m-2·s-1,40 μmol·m-2·s-1,50 μmol·m-2·s-1,60 μmol·m-2·s-1,80μmol·m-2·s-1)at a salinity of 30.The results showed that the free filaments of Py.acanthophora grew fastest at a salinity of 35,and the relative daily growth rate,chlorophyll a,carotenoids,phycoerythrin,phycocyanin and soluble protein contents were all the highest.Furthermore,the daily relative growth rate was highest under the illumination intensity of 30 μmol·m-2·s-1,with the highest contents of chlorophyll a,phycoerythrin and phycocyanin.In conclusion,the optimal growth conditions for free filaments of Py.acanthophora are salinity of 35 and illumination intensity of 30 μmol·m-2·s-1.
Pyropia acanthophorasalinityillumination intensitygrowth and physiologyfree filament
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