In order to explore the optimal salinity and light intensity conditions for the culture of Py.acanthophora free filaments,we induced free filaments from thallus collected from Tongguling,Wenchang,Hainan Province,and crushed them into 150-200 μm segments.They were then placed in conical flasks and cultured for 29 d under the condition of 12L∶12D at 20 ℃.The growth of free filaments were observed under five salinity gradient groups(15,20,25,30,35)at the illumination intensity of 30 μmol·m-2·s-1,and six illumination intensity gradient groups(20 μmol·m-2·s-1,30 μmol·m-2·s-1,40 μmol·m-2·s-1,50 μmol·m-2·s-1,60 μmol·m-2·s-1,80μmol·m-2·s-1)at a salinity of 30.The results showed that the free filaments of Py.acanthophora grew fastest at a salinity of 35,and the relative daily growth rate,chlorophyll a,carotenoids,phycoerythrin,phycocyanin and soluble protein contents were all the highest.Furthermore,the daily relative growth rate was highest under the illumination intensity of 30 μmol·m-2·s-1,with the highest contents of chlorophyll a,phycoerythrin and phycocyanin.In conclusion,the optimal growth conditions for free filaments of Py.acanthophora are salinity of 35 and illumination intensity of 30 μmol·m-2·s-1.
Pyropia acanthophorasalinityillumination intensitygrowth and physiologyfree filament
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