Effects of Different Levels of Dietary Chitooligosaccharides on Growth and Antioxidant Function of Juvenile Yellow River Common Carp
Yellow River common carp(Cyprinus carpio)juveniles with body weight of about 20 g were reared in a 0.4 m3 aquarium in a controllable experimental system for circulating aquaculture,with 30 animals per aquarium,and fed diets containing 0(basal feed,control group),0.02％,0.04％and 0.06％of chitooligosaccharides(molecular weight less than 3 500 Da and 10％purity)for 56 d at water temperature of(25.5±3.0)℃.The contents of serum blood glucose(GLU),total protein(TP),albumin(ALB),triglycerides(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)and malondialdehyde(MDA),and activities of lysozyme,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were determined to probe into the effects of chitooligosaccharides on the growth and antioxidant function of Yellow River common carp.The results showed that the relative growth rates were increased s by 4.43％in 0.04％group and 6.00％in 0.06％group,without significant difference compared with that in the control group(P＞0.05).The hepatopancreatic index,however,was shown to be significantly decreased by 17.5％in 0.04％group and 12.5％in 0.06％group(P＜0.05).The serum lysozyme activity in each test group was increased to some extent with the increase in dietary chitooligosaccharide level compared with the control group.At the same time,the activity of SOD in serum was significantly increased in 0.04％chitooligosaccharide group.There were increase in GSH-Px activities in serum and liver to some extent in chitooligosaccharide groups,without significant difference(P＞0.05),and with significant reduce in serum MDA content in 0.06％chitooligosaccharide group.In conclusion,addition of different concentrations of chitooligosaccharides to basal diet did not lead to significantly affect the growth of the common carp,but to some extent to improve the immune and antioxidant indexes,thus improving the health level of carp.The optimal dose of chitooligosaccharide is recommended to be 0.02％-0.04％in feed based on various indices and economic cost factors.
juvenile Yellow River common carpchitooligosaccharidegrowthantioxidant
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