Effects of Bleaching Powder on Microbial Diversity and Community Structure in Mariculture Disinfection Ponds
To grasp and understand the effect of bleaching powder disinfection on the microbial community structure of aquaculture influent,the microbial diversity and microbial community structure of disinfected water at surface,middle and bottom layers were compared and analyzed for 0h,12 h,24 h and 48 h,using high-throughput sequencing technology.It was found that there was the minimal microbial community richness and diversity in sedimentation,sand filtration and disinfection treatment at 12 h,and the maximal at 48 h.At the level of phylum,the bacteria were showed to be belonged to 18 groups,with the dominant bacteria in Proteobacteria(90.5％),Bacteroidetes(4.07％),Cyanobacteria(2.21％)and Firmicutes(2.11％).Three groups were reduced compared to before disinfection by 24.83％in Proteobacteria,cyanobacteria becoming the non-dominant bacteria(0.02％),and by 11,4 and 6 groups in the bacterial communities at 12 h,24 h and 48 h.At the level of genus,the maximal relative abundance was observed in SAR1 1 unclassified(76.10％),and the abundance of the dominant bacteria was decreased to less than 3％after precipitation and disinfection,with the minimal to 0.09％at 12 h.The relative abundances of the dominant Bacillus and Lactococcus were found to be increased to 36.73％and 26.99％at 12 h respectively,and the dominant bacteria Gammaproteobacteria_unclassified with the relative abundances of 26.8％,the unclassified group others accounting for 21.88％at 24 h.The relative abundance of unclassified group others was 41.03％at 48 h with the increase of disinfection time.The findings indicated that bleaching powder disinfection changed the microbial diversity and community structure of the water environment,and 50 mg/L bleaching powder after sedimentation and sand filtration,should enter the internal circulation of the culture pond in time during 24-48 h,in order to reduce the chances of unknown microorganisms entering the internal circulation.
bleaching powdermariculturemicrobial diversitymicrobial community structure
NETL
NSTL
万方数据
