植物乳杆菌LP-37和氟苯尼考对许氏平鲉(Sebastes schlegelii)肠道形态、消化酶及免疫酶活性的影响

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中文摘要：为分析植物乳杆菌(Lactobacillus plantarum)LP-37和氟苯尼考对许氏平鲉(Sebastes schlegelii)肠道形态的影响,在水温(14±1)℃条件下,将体质量为(32.23±9.38)g的许氏平鲉饲养在约5 m3的循环水圆桶中45 d,分别投喂4种饲料:喷洒去离子水的基础饲料(对照组C0);前30d投喂喷洒1 × 107 CFU/g植物乳杆菌的饲料,后15 d投喂对照饲料(植物乳杆菌组,P1);前30 d投喂喷洒20 mg/L氟苯尼考的饲料,后15 d投喂对照饲料(氟苯尼考组,F1);前15 d投喂喷洒氟苯尼考的饲料,中间15 d投喂喷洒植物乳杆菌的饲料,最后15 d投喂对照饲料(植物乳杆菌和氟苯尼考组,G1).在第30 d和第45 d解剖许氏平鲉的肠道,通过HE染色和Image J软件分析肠道形态结构的变化,并测定血清中α-淀粉酶、脂肪酶、胰蛋白酶、碱性磷酸酶和超氧化物歧化酶活性的变化.结果显示,1 × 107CFU/g植物乳杆菌和20 mg/L的氟苯尼考可以改善肠道结构,显著提高肠道绒毛长度、肌层厚度、隐窝深度(P＜0.05);1 × 107 CFU/g植物乳杆菌LP-37可以显著提高许氏平鲉血清中α-淀粉酶、碱性磷酸酶、超氧化物歧化酶活性(P＜0.05);20 mg/L的氟苯尼考可以显著提高许氏平鲉血清α-淀粉酶、胰蛋白酶活性、碱性磷酸酶、超氧化物歧化酶的活性,显著降低脂肪酶活性(P＜0.05);组合使用1 × 107 CFU/g植物乳杆菌LP-37和20 mg/L氟苯尼考可以显著提高许氏平鲉血清α-淀粉酶、超氧化物歧化酶的活性(P＜0.05),显著降低脂肪酶、胰蛋白酶的活性(P＜0.05).综上所述,植物乳杆菌LP-37和氟苯尼考均可以改善许氏平鲉肠道结构,调节许氏平鲉消化酶和免疫酶的活性.

外文标题：Effects of Lactobacillus plantarum LP-37 and Florfenicol on Intestinal Histomorphology,and Activities of Digestive Enzymes and Immune Enzymes of Schlegel's Black Rockfish Sebastes schlegelii

外文摘要：In order to analyze the effects of Lactobacillus plantarum LP-37 and florfenicol on the intestinal histomorphology of Sebastes schlegelii,with body weight of(32.23±9.38)g were reared in a circulating water tank of about 5 m3 for 45 d at water temperature of(14±1)℃,and fed four diets were fed separately:basal diet sprayed with deionized water(control group,C0);diet with 1× 107 CFU/g L.plantarum was sprayed for 30 d,followed by control diet(P1)for 15 d;diet sprayed with 20 mg/L florfenicol for 30 d,followed by control diet(florfenicol group,F1)for 15 d;diet sprayed with florfenicol for 15 d,then diet sprayed with L.plantarum for 15 d,and then diet with control diet for 15 d(L.plantarum and florfenicol group,G1).The Sebastes schlegelii were dissected and took intestines in the 30th and the 45th day,and the changes of intestinal histomorphological structure were observed by HE staining and Image J software.Changes in α-amylase,lipase,trypsin,alkaline phosphatase,and superoxide dismutase were measured in serum.The results showed that the length of intestinal villi,the thickness of muscular layer and the depth of crypt(P＜0.05)were observed in the fed diets containing 1 × 107 CFU/g L.plantarum and 20 mg/L florfenicol.The activities of α-amylase,alkaline phosphatase and superoxide dismutase were significantly increased in the fed diet containing 1 × 107 CFU/g L.plantarum LP-37 can(P＜0.05).The activity of α-amylase,trypsin,alkaline phosphatase and superoxide dismutase were significantly increased by florfenicol at 20 mg/L,and the activity of lipase was significantly decreased(P＜0.05).Combination 1× 107 CFU/g L.plantarum LP-37 and 20 mg/L florfenicol led to significantly increase α-amylase activity and superoxide dismutase activity(P＜0.05)significantly decreased lipase and trypsin activity(P＜0.05).To sum up,L.plantarum LP-37 and florfenicol improved the intestinal structure of Sebastes schlegelii and regulated the digestive and immune enzyme activities of Sebastes schlegelii.

外文关键词：

Sebastes schlegeliiintestinal morphologyimmune enzymedigestive enzymeLactobacillus plantarumflorfenicol

作者：

宋德青、于晓清、季相山、李莉、王友红、王晓璐、许拉、盖春蕾、樊英、叶海斌

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作者单位：

山东省海洋科学研究院(青岛国家海洋科学研究中心),山东省海水养殖病害防治重点实验室,青岛市水产生物品质评价与利用工程研究中心,山东青岛 266104

山东农业大学,山东泰安 271018

关键词：

许氏平鲉肠道形态消化酶免疫酶植物乳杆菌氟苯尼考

基金：

山东省鱼类产业技术体系建设项目海洋科技创新专项海洋科技创新示范工程项目(2023)山东省重点研发计划科技示范工程项目

项目编号：

SDAIT-12-0123-1-3-hysf-2-hy2021SFGC0701

出版年：

2024

水产学杂志

中国水产科学研究院黑龙江水产研究所

水产学杂志

影响因子：0.497

ISSN：1005-3832

年,卷(期)：2024.37(4)

参考文献量12