The protoplasts of Saccharina japonica were prepared and collected from kelp sporophytes which were cultivated at sea for 3-5months to address the identification needs in kelp polyploidy breeding.The result showed that two types of protoplasts with different diameters were obtained after enzymatic hydrolysis of the growth tissue of kelp sporophytes,which meet the requirement for DNA content and ploidy detection by Flow Cytometry.Then,they were tested using Flow Cytometry after cell dissociation and fluorescence staining.A Flow Cytometry histogram would be obtained that can be used to estimate DNA content and then identify the ploidy of S.japonica sporophyte.The research has indicated that the Flow Cytometry method for identifying the ploidy is suitable for preliminary ploidy identification of kelp sporophytes.
维普
万方数据