The study of Fu Huang Zhi Yang Tang inhibits IL-31/TRPV1/ERK1/2 signal axis and alleviates skin pruritus in uremic mice
Objective:To elucidate the efficacy of Fu Huang Zhi Yang Tang on Uremic Pruritus and its mechanism of action.Methods:40mice were randomly divided into 5groups(Sham,Model,FHZYT,BCTC,FHZYT+BCTC),8mice in each group,and the number of scratching was counted after the intervention was completed.Indicator tests included immunohistochemical stai-ning to detect the expression of TNF-α,IL-1β,Substance P and IL-31 in the skin.WB to detect the protein expression of Sub-stance P,TNF-α,IL-1β,IL-31,TRPV1,ERK1/2 and p-ERK1/2 in the skin.Results:1.Mice in the Model group had in-creased levels of creatinine and urea nitrogen,and the number of itching increased.2The expression of TNF-α and IL-1 β was up-regulated in the Model group,whereas the expression of TNF-α was decreased in the FHZYT,BCTC,and FHZYT+BCTC groups.3Compared with the Model group,the number of itching in the FHZYT,BCTC,and FHZYT+BCTC groups was relatively The expression levels of substance P,IL-31,TRPV1,ERK1/2 and p-ERK1/2 in the skin were reduced.Conclusion:Fu Huang Zhi Yang Tang can effectively reduce itching in uremic mice,and its mechanism may be through the inhibition of IL-31/TRPV1/ERK1/2 signalling axis,which improves the inflammatory state of the skin and exerts the effect of itching relief.
Uremic PruritusFu Huang Zhi Yang TangIL-31TRPV1ERK1/2
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